NuSAP4 regulates chromosome segregation in Trypanosoma brucei by promoting bipolar spindle assembly.

IF 5.2 1区生物学 Q1 BIOLOGY Communications Biology Pub Date : 2024-11-16 DOI:10.1038/s42003-024-07248-5

Qing Zhou, Ziyin Li

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Abstract

Faithful chromosome segregation in eukaryotes requires the assembly of a bipolar spindle and the faithful attachment of kinetochores to spindle microtubules, which are regulated by various spindle-associated proteins (SAPs) that play distinct functions in regulating spindle dynamics and microtubule-kinetochore attachment. The protozoan parasite Trypanosoma brucei employs evolutionarily conserved and kinetoplastid-specific proteins, including some kinetoplastid-specific nucleus- and spindle-associated proteins (NuSAPs), to regulate chromosome segregation. Here, we characterized NuSAP4 and its functional interplay with diverse SAPs in promoting chromosome segregation in T. brucei. NuSAP4 associates with the spindle during mitosis and concentrates at spindle poles where it interacts with SPB1 and MAP103. Knockdown of NuSAP4 impairs chromosome segregation by disrupting bipolar spindle assembly and spindle pole protein localization. These results uncover the mechanistic role of NuSAP4 in regulating chromosome segregation by promoting bipolar spindle assembly, and highlight the unusual features of mitotic regulation by spindle-associated proteins in this early divergent microbial eukaryote.

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NuSAP4 通过促进双极纺锤体的组装来调节布氏锥虫的染色体分离。

真核生物染色体的忠实分离需要双极纺锤体的组装以及动点系与纺锤体微管的忠实附着，而这些都受到各种纺锤体相关蛋白（SAP）的调控，这些蛋白在调控纺锤体动力学和微管-动点系附着方面发挥着不同的功能。原生动物布氏锥虫（Trypanosoma brucei）利用进化保守和动点细胞特异性蛋白（包括一些动点细胞特异性细胞核和纺锤体相关蛋白（NuSAPs））来调节染色体分离。在这里，我们对 NuSAP4 及其在促进布氏原虫染色体分离过程中与多种 SAP 的功能相互作用进行了表征。NuSAP4 在有丝分裂过程中与纺锤体结合，并集中在纺锤体两极，与 SPB1 和 MAP103 相互作用。通过破坏双极纺锤体组装和纺锤极蛋白定位，敲除NuSAP4会影响染色体分离。这些结果揭示了NuSAP4通过促进双极纺锤体组装来调节染色体分离的机制作用，并突出了这种早期分化的微生物真核细胞中纺锤体相关蛋白对有丝分裂调节的不寻常特征。

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来源期刊

Communications Biology Medicine-Medicine (miscellaneous)

CiteScore

8.60

自引率

1.70%

发文量

1233

审稿时长

13 weeks

期刊介绍： Communications Biology is an open access journal from Nature Research publishing high-quality research, reviews and commentary in all areas of the biological sciences. Research papers published by the journal represent significant advances bringing new biological insight to a specialized area of research.