Dheeraj K Agrohia, Ritabrita Goswami, Yağız Anıl Çiçek, Neslihan E Tabaru, Trisha W Brady, Vincent M Rotello, Richard W Vachet
{"title":"Multiplexed Quantitative Screening of the Cellular Uptake of Proteins Delivered by Polymeric Nanocarriers.","authors":"Dheeraj K Agrohia, Ritabrita Goswami, Yağız Anıl Çiçek, Neslihan E Tabaru, Trisha W Brady, Vincent M Rotello, Richard W Vachet","doi":"10.1021/acs.analchem.4c05191","DOIUrl":null,"url":null,"abstract":"<p><p>Proteins are important therapeutic agents, yet better methods are needed to deliver them inside of cells. Polymeric nanocarriers (PNCs) are versatile materials for this purpose, and to enhance their development, it is necessary to quantify protein delivery efficiency into cells by numerous PNC designs at the same time. Current strategies for screening PNC systems are qualitative and mostly serial. Here, we describe a multiplexed approach that uses metal-coded mass tags (MMTs) to quantify the delivery of protein into cells by several different PNC designs simultaneously. Our approach will facilitate the development of more potent delivery systems by improving precision and reducing costs, effort, and time.</p>","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":" ","pages":"18922-18926"},"PeriodicalIF":6.7000,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.4c05191","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/18 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Proteins are important therapeutic agents, yet better methods are needed to deliver them inside of cells. Polymeric nanocarriers (PNCs) are versatile materials for this purpose, and to enhance their development, it is necessary to quantify protein delivery efficiency into cells by numerous PNC designs at the same time. Current strategies for screening PNC systems are qualitative and mostly serial. Here, we describe a multiplexed approach that uses metal-coded mass tags (MMTs) to quantify the delivery of protein into cells by several different PNC designs simultaneously. Our approach will facilitate the development of more potent delivery systems by improving precision and reducing costs, effort, and time.
期刊介绍：
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
