CLCA1 and BPIFB1 are potential novel biomarkers for asthma: an iTRAQ analysis.

IF 2.1 3区 医学 Q3 RESPIRATORY SYSTEM Journal of thoracic disease Pub Date : 2024-10-31 Epub Date: 2024-10-28 DOI:10.21037/jtd-24-1366
Tianci Chai, Yinji Liu, Yuwei Zeng, Sung-Yoon Kang, Jie Li
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Abstract

Background: Asthma is a chronic respiratory disease that affects billions of people. Due to its diverse phenotypes and endotypes with distinct pathophysiological mechanisms, significant challenges arise in its clinical diagnosis and treatment. The discovery of potential biomarkers of asthma has significant implications for its clinical classification and precise treatment. The purpose of this study is to identify potential biomarkers for asthma, providing a foundation for its diagnosis and treatment.

Methods: We constructed an ovalbumin (OVA)-sensitized asthmatic mice model and used isobaric Tags for Relative and Absolute Quantitation (iTRAQ) labeling and liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) technology to identify differentially expressed proteins (DEPs) in lung tissues. We then performed enrichment analyses of the DEPs using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, and constructed protein-protein interaction (PPI) networks.

Results: We identified 242 DEPs in the asthmatic mice model and showed that heat shock protein family A (Hsp70) member 5 (HSPA5) is a central protein in asthma. Consistent with our bioinformatics analysis, our western blot validation confirmed that the protein levels of arginase 1 (ARG1), chitinase-like protein 3 (CHIL3), chloride channel accessory 1 (CLCA1), and bactericidal/permeability-increasing protein (BPI) fold-containing family B member 1 (BPIFB1) were significantly increased in asthma group compared to the control group. Thus, we found that CLCA1 and BPIFB1 were the most promising potential biomarkers of asthma.

Conclusions: Our iTRAQ analysis and western blot verification of asthmatic mice showed that HSPA5 is a central protein in asthma, and CLCA1 and BPIFB1 are novel potential biomarkers that could play significant roles in the diagnosis and treatment of asthma.

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CLCA1和BPIFB1是哮喘的潜在新型生物标记物:一项iTRAQ分析。
背景:哮喘是一种影响数十亿人的慢性呼吸道疾病。由于哮喘的表型和内型多种多样,病理生理机制也各不相同,因此临床诊断和治疗面临着巨大的挑战。发现哮喘的潜在生物标志物对其临床分类和精确治疗具有重要意义。本研究旨在确定哮喘的潜在生物标志物,为哮喘的诊断和治疗奠定基础:方法:我们构建了卵清蛋白(OVA)致敏的哮喘小鼠模型,并使用等位相对绝对定量标记(iTRAQ)和液相色谱-质谱/质谱(LC-MS/MS)技术鉴定了肺组织中的差异表达蛋白(DEPs)。然后,我们利用基因本体(GO)和京都基因与基因组百科全书(KEGG)数据库对DEPs进行了富集分析,并构建了蛋白质-蛋白质相互作用(PPI)网络:结果:我们在哮喘小鼠模型中发现了242个DEPs,并发现热休克蛋白A家族(Hsp70)成员5(HSPA5)是哮喘的核心蛋白。与我们的生物信息学分析一致,我们的Western印迹验证证实,与对照组相比,哮喘组中精氨酸酶1(ARG1)、几丁质酶样蛋白3(CHIL3)、氯离子通道附属物1(CLCA1)和杀菌/渗透性增强蛋白(BPI)折叠包含家族B成员1(BPIFB1)的蛋白水平显著升高。因此,我们发现 CLCA1 和 BPIFB1 是最有希望的哮喘潜在生物标记物:结论:我们对哮喘小鼠的 iTRAQ 分析和 Western 印迹验证表明,HSPA5 是哮喘的中心蛋白,而 CLCA1 和 BPIFB1 是新的潜在生物标记物,可在哮喘的诊断和治疗中发挥重要作用。
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来源期刊
Journal of thoracic disease
Journal of thoracic disease RESPIRATORY SYSTEM-
CiteScore
4.60
自引率
4.00%
发文量
254
期刊介绍: The Journal of Thoracic Disease (JTD, J Thorac Dis, pISSN: 2072-1439; eISSN: 2077-6624) was founded in Dec 2009, and indexed in PubMed in Dec 2011 and Science Citation Index SCI in Feb 2013. It is published quarterly (Dec 2009- Dec 2011), bimonthly (Jan 2012 - Dec 2013), monthly (Jan. 2014-) and openly distributed worldwide. JTD received its impact factor of 2.365 for the year 2016. JTD publishes manuscripts that describe new findings and provide current, practical information on the diagnosis and treatment of conditions related to thoracic disease. All the submission and reviewing are conducted electronically so that rapid review is assured.
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