The new ice age: the promise and challenges of rapid oocyte warming protocols.

Abstract

Oocyte cryopreservation has transformed assisted reproductive technology, providing enhanced flexibility and efficacy in assisted reproductive technology treatments. The shift from traditional slow freezing to vitrification has markedly improved survival rates by preventing ice crystal formation, which can damage cellular structures. Despite these advances, rapid warming of vitrified oocytes remains a challenge due to the unique sensitivity of oocytes to thermal and osmotic stress, which can compromise spindle integrity and developmental competence. Recent studies indicate that using both rapid vitrification and rapid warming protocols may reduce processing time and enhance workflow efficiency, yet the impact of rapid warming on oocytes previously cryopreserved using standard methods is not fully understood. Although promising results have emerged from preclinical and clinical studies using rapid warming on embryos, the applicability to human oocytes requires further research. For oocytes, establishing a universal rapid warming protocol that balances efficiency and safety is essential, especially considering the millions of oocytes already preserved with conventional techniques.