Broad-scale phenotyping in Arabidopsis reveals varied involvement of RNA interference across diverse plant-microbe interactions.

IF 2.3 3区生物学 Q2 PLANT SCIENCES Plant Direct Pub Date : 2024-11-15 eCollection Date: 2024-11-01 DOI:10.1002/pld3.70017

Alessa Ruf, Hannah Thieron, Sabrine Nasfi, Bernhard Lederer, Sebastian Fricke, Trusha Adeshara, Johannes Postma, Patrick Blumenkamp, Seomun Kwon, Karina Brinkrolf, Michael Feldbrügge, Alexander Goesmann, Julia Kehr, Jens Steinbrenner, Ena Šečić, Vera Göhre, Arne Weiberg, Karl-Heinz Kogel, Ralph Panstruga, Silke Robatzek

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Abstract

RNA interference (RNAi) is a crucial mechanism in immunity against infectious microbes through the action of DICER-LIKE (DCL) and ARGONAUTE (AGO) proteins. In the case of the taxonomically diverse fungal pathogen Botrytis cinerea and the oomycete Hyaloperonospora arabidopsidis, plant DCL and AGO proteins have proven roles as negative regulators of immunity, suggesting functional specialization of these proteins. To address this aspect in a broader taxonomic context, we characterized the colonization pattern of an informative set of DCL and AGO loss-of-function mutants in Arabidopsis thaliana upon infection with a panel of pathogenic microbes with different lifestyles, and a fungal mutualist. Our results revealed that, depending on the interacting pathogen, AGO1 acts as a positive or negative regulator of immunity, while AGO4 functions as a positive regulator. Additionally, AGO2 and AGO10 positively modulated the colonization by a fungal mutualist. Therefore, analyzing the role of RNAi across a broader range of plant-microbe interactions has identified previously unknown functions for AGO proteins. For some pathogen interactions, however, all tested mutants exhibited wild-type-like infection phenotypes, suggesting that the roles of AGO and DCL proteins in these interactions may be more complex to elucidate.

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拟南芥的大范围表型分析表明，RNA 干扰在植物与微生物的多种相互作用中发挥着不同的作用。

RNA 干扰（RNAi）是通过 DICER-LIKE 蛋白（DCL）和 ARGONAUTE 蛋白（AGO）的作用对传染性微生物进行免疫的重要机制。在分类学上多种多样的真菌病原体 Botrytis cinerea 和卵菌 Hyaloperonospora arabidopsidis 中，植物 DCL 和 AGO 蛋白已被证明具有免疫负调控因子的作用，这表明这些蛋白具有功能特异性。为了在更广泛的分类背景下解决这方面的问题，我们研究了拟南芥中一组信息丰富的 DCL 和 AGO 功能缺失突变体在感染具有不同生活方式的病原微生物和真菌互惠体时的定殖模式。我们的研究结果表明，根据相互作用的病原体，AGO1 是免疫的正向或负向调节因子，而 AGO4 则是正向调节因子。此外，AGO2 和 AGO10 对真菌互生体的定殖有正向调节作用。因此，通过分析 RNAi 在更广泛的植物与微生物相互作用中的作用，发现了 AGO 蛋白以前未知的功能。然而，对于某些病原体相互作用，所有测试的突变体都表现出类似野生型的感染表型，这表明 AGO 蛋白和 DCL 蛋白在这些相互作用中的作用可能更为复杂，有待阐明。

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来源期刊

Plant Direct Environmental Science-Ecology

CiteScore

5.00

自引率

3.30%

发文量

101

审稿时长

14 weeks

期刊介绍： Plant Direct is a monthly, sound science journal for the plant sciences that gives prompt and equal consideration to papers reporting work dealing with a variety of subjects. Topics include but are not limited to genetics, biochemistry, development, cell biology, biotic stress, abiotic stress, genomics, phenomics, bioinformatics, physiology, molecular biology, and evolution. A collaborative journal launched by the American Society of Plant Biologists, the Society for Experimental Biology and Wiley, Plant Direct publishes papers submitted directly to the journal as well as those referred from a select group of the societies’ journals.