Efficacy of ozone versus mesenchymal stem cell-derived microvesicles in ameliorating testicular changes after hypothyroidism in adult albino rats: a histological and immunohistochemical study.

IF 1.1 4区 医学 Q4 MICROSCOPY Ultrastructural Pathology Pub Date : 2024-11-01 Epub Date: 2024-11-17 DOI:10.1080/01913123.2024.2423863
Magdy F Gawish, Samia A Abd El-Baset, Salma S Shalabi, Nahla E Ibrahem
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Abstract

This study was performed to: detect the histological, immunohistochemical, and biochemical alterations that may occur in the testes of adult rats in induced hypothyroidism. And to investigate which one, ozone or MSCs-MVs have better therapeutic effect on testicular changes after hypothyroidism. Eighty-four male adult rats were separated into: control group, hypothyroidism group: rats will be given carbimazole for 30 days, ozone group: rats treated as hypothyroidism group then will be injected with ozone intraperitoneal for 7 days. MSC-MVs group: rats treated as hypothyroidism group then will be injected with a single intravenous dose MSC-MVs. Specimens of testes were handled for light, electron microscope, and immunohistochemical of vimentin and S100. Biochemical analysis for; MDA and TNFα; serum testosterone, TSH, T3, and T4 was done, also, sperm count and morphology assay. Morphometric and statistical analysis were performed. Hypothyroidism group showed disorganized seminiferous tubules. A noticeable gap was between the basement membrane and the germinal epithelium. Wide interstitium had congested vessels and acidophilic homogenous material. Vacuolated germinal epithelium and few germ cells had dark nuclei with noticeable separation of between the basement membrane and the germinal epithelium. Ozone and MSCs-MVs induced improvement in all the previous parameters and restoration of spermatogenesis. In Conclusion MSCs-MVs has better ameliorative effect than ozone on hypothyroidism-exposed testes.

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臭氧与间充质干细胞衍生的微囊在改善成年白化大鼠甲状腺功能减退后睾丸变化方面的功效:组织学和免疫组织化学研究。
本研究旨在:检测甲状腺功能减退症诱导的成年大鼠睾丸可能发生的组织学、免疫组化和生化改变。并研究臭氧和间充质干细胞-间充质干细胞对甲状腺机能减退后睾丸变化的治疗效果。将84只雄性成年大鼠分为对照组、甲减组(给予卡比马唑治疗30天)、臭氧组(在甲减组基础上腹腔注射臭氧7天)。间充质干细胞-间充质干细胞组:大鼠接受甲状腺功能减退组治疗后,静脉注射单剂量间充质干细胞-间充质干细胞。对睾丸标本进行光镜、电子显微镜检查,并对波形蛋白和 S100 进行免疫组化。生化分析包括 MDA 和 TNFα、血清睾酮、促甲状腺激素、T3 和 T4,以及精子计数和形态学检测。进行了形态计量和统计分析。甲状腺机能减退组显示出曲细精管紊乱。基底膜和生精上皮之间有明显的间隙。宽大的间质中有充血的血管和嗜酸性均质物质。空泡化的生殖上皮和少数生殖细胞的核呈深色,基底膜和生殖上皮之间有明显的分隔。臭氧和间充质干细胞-间充质干细胞可改善之前的所有参数,恢复精子发生。结论间充质干细胞-间充质干细胞比臭氧对甲状腺功能减退症暴露的睾丸有更好的改善作用。
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来源期刊
Ultrastructural Pathology
Ultrastructural Pathology 医学-病理学
CiteScore
2.00
自引率
10.00%
发文量
40
审稿时长
6-12 weeks
期刊介绍: Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.
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