Emerging biotechnologies for engineering liver organoids

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL Bioactive Materials Pub Date : 2024-11-08 DOI:10.1016/j.bioactmat.2024.11.002

Junqi Zhao , Yue Zhi , Haozhen Ren , Jinglin Wang , Yuanjin Zhao

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Abstract

The engineering construction of the liver has attracted enormous attention. Organoids, as emerging miniature three-dimensional cultivation units, hold significant potential in the biomimetic simulation of liver structure and function. Despite notable successes, organoids still face limitations such as high variability and low maturity. To overcome these challenges, engineering strategies have been established to maintain organoid stability and enhance their efficacy, laying the groundwork for the development of advanced liver organoids. The present review comprehensively summarizes the construction of engineered liver organoids and their prospective applications in biomedicine. Initially, we briefly present the latest research progress on matrix materials that maintain the three-dimensional morphology of organoids. Next, we discuss the manipulative role of engineering technologies in organoid assembly. Additionally, we outline the impact of gene-level regulation on organoid growth and development. Further, we introduce the applications of liver organoids in disease modeling, drug screening and regenerative medicine. Lastly, we overview the current obstacles and forward-looking perspectives on the future of engineered liver organoids. We anticipate that ongoing innovations in engineered liver organoids will lead to significant advancements in medical applications.

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用于肝脏器官组织工程的新兴生物技术

肝脏的工程构造引起了极大的关注。有机体作为新兴的微型三维培养单元，在肝脏结构和功能的生物仿真模拟方面具有巨大潜力。尽管取得了显著的成功，但器官组织仍然面临着变异性大和成熟度低等限制。为了克服这些挑战，人们建立了工程策略来保持类器官的稳定性并提高其功效，从而为开发先进的肝脏类器官奠定了基础。本综述全面总结了工程肝脏器官组织的构建及其在生物医学中的应用前景。首先，我们简要介绍了保持器官组织三维形态的基质材料的最新研究进展。接着，我们讨论了工程技术在类器官组装中的操纵作用。此外，我们还概述了基因水平调控对类器官生长和发育的影响。此外，我们还介绍了肝脏类器官在疾病建模、药物筛选和再生医学中的应用。最后，我们概述了工程肝脏器官组织目前面临的障碍和对未来的前瞻性展望。我们预计，工程肝脏器官组织的不断创新将为医学应用带来重大进展。

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来源期刊

Bioactive Materials Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

28.00

自引率

6.30%

发文量

436

审稿时长

20 days

期刊介绍： Bioactive Materials is a peer-reviewed research publication that focuses on advancements in bioactive materials. The journal accepts research papers, reviews, and rapid communications in the field of next-generation biomaterials that interact with cells, tissues, and organs in various living organisms. The primary goal of Bioactive Materials is to promote the science and engineering of biomaterials that exhibit adaptiveness to the biological environment. These materials are specifically designed to stimulate or direct appropriate cell and tissue responses or regulate interactions with microorganisms. The journal covers a wide range of bioactive materials, including those that are engineered or designed in terms of their physical form (e.g. particulate, fiber), topology (e.g. porosity, surface roughness), or dimensions (ranging from macro to nano-scales). Contributions are sought from the following categories of bioactive materials: Bioactive metals and alloys Bioactive inorganics: ceramics, glasses, and carbon-based materials Bioactive polymers and gels Bioactive materials derived from natural sources Bioactive composites These materials find applications in human and veterinary medicine, such as implants, tissue engineering scaffolds, cell/drug/gene carriers, as well as imaging and sensing devices.