Biofabrication, characterization and unveiling the biological potential of zinc oxide nanoparticles from bacterium Bacillus pacificus KR A1

IF 5.7 2区材料科学 Q2 CHEMISTRY, PHYSICAL Surfaces and Interfaces Pub Date : 2024-11-02 DOI:10.1016/j.surfin.2024.105382

Durairaj Karthick Rajan , Jinzheng Wu , Hao Li , Kaitian Zhang , Shubing Zhang

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Abstract

The utilization of cell free supernatant for synthesizing nanomaterials has been considered as an innovative approach. This investigation aims to fabricate a ZnO NPs using the cell free supernatant of Bacillus pacificus KR A1. The prepared nanomaterials were systematically characterized by UV-DRS, FT-IR, XRD, HR-TEM, XPS and elemental mapping (EDX) techniques. The optical band gap energy of Bacillus pacificus ZnO NPs (Bp-ZnO NPs) was observed as 3.25 eV. The XRD analysis revealed that Bp-ZnO NPs were hexagonal wurtzite structures with 50 nm crystallite size. Furthermore, XPS data revealed the elements present in Bp-ZnO NPs. The HR-TEM analysis revealed that most of the particles were spherical in shape with an average calculated particle size of 20 nm with a lattice fringe range of 0.246 nm. In addition, Bp-ZnO NPs showed promising antibiofilm properties against Bacillus cereus and Vibrio alginolyticus at 100 μg/mL concentration. Furthermore, MDA-MB-231 cells treated with an IC₅₀ concentration showed changes in cell morphology including apoptosis, cell shrinkage and nuclear fragmentation. However, HUVEC cells treated with higher concentration (40 µg/mL) of Bp-ZnO NPs displayed less toxicity and 86.3 % of cells are viable. In summary, Bp-ZnO NPs could serve as a promising material for biomedical claims.

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太平洋芽孢杆菌 KR A1 纳米氧化锌的生物制造、表征和生物潜力揭示

利用细胞游离上清液合成纳米材料被认为是一种创新方法。本研究旨在利用太平洋芽孢杆菌 KR A1 的细胞游离上清液制备氧化锌纳米粒子。制备的纳米材料通过 UV-DRS、FT-IR、XRD、HR-TEM、XPS 和元素图谱（EDX）技术进行了系统表征。观察到太平洋芽孢杆菌氧化锌纳米粒子（Bp-ZnO NPs）的光带隙能为 3.25 eV。XRD 分析表明，Bp-ZnO NPs 为六方菱面体结构，晶粒大小为 50 纳米。此外，XPS 数据还揭示了 Bp-ZnO NPs 中存在的元素。HR-TEM 分析表明，大多数颗粒呈球形，平均计算粒度为 20 纳米，晶格边缘范围为 0.246 纳米。此外，在 100 μg/mL 浓度下，Bp-ZnO NPs 对蜡样芽孢杆菌和藻溶弧菌具有良好的抗生物膜特性。此外，以 IC50 浓度处理的 MDA-MB-231 细胞显示出细胞形态的变化，包括细胞凋亡、细胞萎缩和核破碎。然而，用较高浓度（40 微克/毫升）的 Bp-ZnO NPs 处理的 HUVEC 细胞毒性较小，86.3% 的细胞存活。总之，Bp-ZnO NPs 可以作为一种很有前景的材料用于生物医学领域。

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来源期刊

Surfaces and Interfaces Chemistry-General Chemistry

CiteScore

8.50

自引率

6.50%

发文量

753

审稿时长

35 days

期刊介绍： The aim of the journal is to provide a respectful outlet for ''sound science'' papers in all research areas on surfaces and interfaces. We define sound science papers as papers that describe new and well-executed research, but that do not necessarily provide brand new insights or are merely a description of research results. Surfaces and Interfaces publishes research papers in all fields of surface science which may not always find the right home on first submission to our Elsevier sister journals (Applied Surface, Surface and Coatings Technology, Thin Solid Films)