Cytotoxic properties of Thuya occidentalis hydroalcoholic extract on androgen unresponsive prostate cancer cells.

IF 2.5 4区医学 Q3 ENDOCRINOLOGY & METABOLISM Archives of Physiology and Biochemistry Pub Date : 2024-11-20 DOI:10.1080/13813455.2024.2430488

Gabriela Elisa Hirsch, Mariana Migliorini Parisi, Leo Anderson Meira Martins, Lílian Corrêa Costa-Beber, Cláudia Marlise Balbinotti Andrade, Florencia Mária Barbé Tuana, Silvia Resende Terra, Tassiane Dos Santos Ferrão, Roger Wagner, Tatiana Emanuelli, Fátima Theresinha Costa Rodrigues Guma

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Abstract

Background: Androgen independent phase in prostate cancer (PCa) commonly limits the therapeutic efficacy. Thuya occidentalis through its main active compound, α-thujone, appears to be an option, considering its anti-proliferative, anti-metastatic and pro-apoptotic effects on hepatocellular carcinoma. However, studies on PCa are limited.

Objective: To evaluate if T. occidentalis could be useful against androgen responsive and unresponsive PCa cells.

Methods: Androgen responsive (LNCaP) and unresponsive (DU145 and PC3) cell lines were exposed to T. occidentalis hydroalcoholic extract (0.05 mL/mL) for different periods. Further, α-thujone was measured in the extract and tested in the cell lines.

Results: T. occidentalis and α-thujone showed the highest cytotoxicity on LNCaP cells. In androgen unresponsive cells, T. occidentalis decreased cell viability and density, and promoted apoptosis, necrosis and cell cycle arrest, possibly associated with Cav-1 downregulation. The α-thujone present in the extract significantly LNCaP cells density, but did not affect DU145 and PC3 cells, suggesting that other compounds may also be cytotoxic to androgen unresponsive cells.

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Thuya occidentalis 水醇提取物对雄激素无反应性前列腺癌细胞的细胞毒性特性。

背景：前列腺癌（PCa）的雄激素独立期通常限制了治疗效果。考虑到其对肝细胞癌的抗增殖、抗转移和促凋亡作用，西番莲的主要活性化合物α-thujone似乎是一种选择。然而，对 PCa 的研究还很有限：目的：评估 T. occidentalis 对雄激素反应性和无反应性 PCa 细胞是否有用：方法：将对雄激素有反应的细胞系（LNCaP）和无反应的细胞系（DU145 和 PC3）暴露于不同时间段的欧芹水醇提取物（0.05 mL/mL）中。此外，还测量了提取物中的α-thujone，并在细胞系中进行了测试：结果：西番莲提取物和 α-thujone 对 LNCaP 细胞的细胞毒性最高。在对雄激素无反应的细胞中，T. occidentalis 可降低细胞活力和密度，促进细胞凋亡、坏死和细胞周期停滞，这可能与 Cav-1 下调有关。提取物中的α-thujone能显著降低LNCaP细胞的密度，但对DU145和PC3细胞没有影响，这表明其他化合物也可能对雄激素无反应细胞具有细胞毒性。

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来源期刊

Archives of Physiology and Biochemistry ENDOCRINOLOGY & METABOLISM-PHYSIOLOGY

CiteScore

6.90

自引率

3.30%

发文量

期刊介绍： Archives of Physiology and Biochemistry: The Journal of Metabolic Diseases is an international peer-reviewed journal which has been relaunched to meet the increasing demand for integrated publication on molecular, biochemical and cellular aspects of metabolic diseases, as well as clinical and therapeutic strategies for their treatment. It publishes full-length original articles, rapid papers, reviews and mini-reviews on selected topics. It is the overall goal of the journal to disseminate novel approaches to an improved understanding of major metabolic disorders. The scope encompasses all topics related to the molecular and cellular pathophysiology of metabolic diseases like obesity, type 2 diabetes and the metabolic syndrome, and their associated complications. Clinical studies are considered as an integral part of the Journal and should be related to one of the following topics: -Dysregulation of hormone receptors and signal transduction -Contribution of gene variants and gene regulatory processes -Impairment of intermediary metabolism at the cellular level -Secretion and metabolism of peptides and other factors that mediate cellular crosstalk -Therapeutic strategies for managing metabolic diseases Special issues dedicated to topics in the field will be published regularly.