Diagnostic value of nanopore sequencing of cerebrospinal fluid samples in tuberculous meningitis.

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES Diagnostic microbiology and infectious disease Pub Date : 2024-11-12 DOI:10.1016/j.diagmicrobio.2024.116593

Chunyan Zhao, Chang Song, Shixiong Yang, Aichun Huang, Yanrong Lin, Chaoyan Xu, Xiaoying Wei, Chunmei Zeng, Yiyi Lan, Xiaoyang Luo, Qingdong Zhu

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Abstract

Objective: To investigate the diagnostic efficacy of nanopore sequencing technology in tuberculous meningitis (TBM).

Methods: Cerebrospinal fluid samples were collected from patients for acid-fast staining microscopy, Mycobacterium tuberculosis solid culture, DNA detection, and nanopore sequencing. Lastly, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and area under the receiver operating characteristic curve (AUC) were calculated and compared among these detection methods.

Results: In this study, 30 TBM patients and 18 non-TBM patients were included. Nanopore sequencing showed higher sensitivity (43.30 %) and AUC (0.661) compared to the other methods. Combining nanopore sequencing and imaging achieved the highest diagnostic performance with sensitivity (60.00 %), specificity (88.90 %), PPV (90.00 %), NPV (57.10 %), and AUC (0.744).

Conclusion: Nanopore sequencing demonstrated superior diagnostic accuracy for TBM, outperforming acid-fast staining, Mycobacterium tuberculosis culture, and DNA detection. When combined with imaging, nanopore sequencing significantly enhanced diagnostic sensitivity and accuracy for TBM.

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结核性脑膜炎脑脊液样本纳米孔测序的诊断价值。

目的：研究纳米孔测序技术在结核性脑膜炎（TBM）中的诊断效果：研究纳米孔测序技术在结核性脑膜炎（TBM）中的诊断效果：方法：采集患者脑脊液样本，进行酸性染色显微镜检查、结核分枝杆菌固体培养、DNA检测和纳米孔测序。最后，计算灵敏度、特异性、阳性预测值（PPV）、阴性预测值（NPV）和接收者工作特征曲线下面积（AUC），并对这些检测方法进行比较：本研究共纳入了 30 名 TBM 患者和 18 名非 TBM 患者。与其他方法相比，纳米孔测序显示出更高的灵敏度（43.30%）和AUC（0.661）。将纳米孔测序与成像技术相结合可获得最高的诊断性能，灵敏度（60.00 %）、特异性（88.90 %）、PPV（90.00 %）、NPV（57.10 %）和AUC（0.744）：纳米孔测序对结核分枝杆菌的诊断准确性优于酸性染色、结核分枝杆菌培养和 DNA 检测。纳米孔测序与成像技术相结合，可显著提高TBM的诊断灵敏度和准确性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.