Huazhi Rougan Granule Alleviates Liver and Intestinal Damage in Non-Alcoholic Fatty Liver Disease by Regulating miR-122 Expression and TLR4/MyD88/NF-κB Pathway Activation.

IF 1.6 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS Combinatorial chemistry & high throughput screening Pub Date : 2024-11-19 DOI:10.2174/0113862073290372240603090844

Ping Xie, Xiaowei Jin, Chan Li, Kun Lv, Ming Deng

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Abstract

Purpose: miR-122 is upregulated in non-alcoholic fatty liver disease (NAFLD) liver tissue, and knockdown of miR-122 protects hepatocytes from lipid metabolism disorders. This study aimed to investigate whether Huazhi Rougan Granule (HRG) alleviates NAFLD liver and intestinal injury by regulating the miR-122-mediated TLR4/MyD88/NF-κB pathway.

Methods: Rats with NAFLD were constructed by high-fat feeding. Serum levels of total cholesterol (TC), triglycerides (TG), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were measured using a fully automated biochemical instrument. Histopathological changes in the liver and small intestine were observed by HE staining. QRT-PCR detected the expression level of miR-122 in the liver tissues. The protein expression of TLR4, MyD88, NF- κB p65, and p-p65 in liver tissues was detected by western blotting.

Results: HRG slowed down the weight gain of NAFLD rats, decreased (P<0.05) the levels of TC, TG, ALT, AST, TNF-α, IL-1β, IL-6, LPS, and Hpt, improved the pathological status of liver and small intestine tissues, upregulated (P<0.05) the expression of ZO-1 and Occludin, downregulated (P<0.05) the protein expression of TLR4, MyD88, and p-p65, and inhibited (P<0.05) the expression of miR-122.

Conclusion: HRG may alleviate hepatic and intestinal injuries in rats with NAFLD by regulating the miR-122-mediated TLR4/MyD88/NF-κB pathway.

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华枝甘露颗粒通过调节miR-122表达和TLR4/MyD88/NF-κB通路活化减轻非酒精性脂肪肝的肝脏和肠道损伤

目的：miR-122在非酒精性脂肪肝（NAFLD）肝组织中上调，敲除miR-122可保护肝细胞免受脂质代谢紊乱的影响。本研究旨在探讨华枝甘露颗粒（HRG）是否能通过调节miR-122介导的TLR4/MyD88/NF-κB通路减轻非酒精性脂肪肝肝脏和肠道损伤：方法：通过高脂喂养构建非酒精性脂肪肝大鼠。使用全自动生化仪测量血清总胆固醇（TC）、甘油三酯（TG）、天冬氨酸氨基转移酶（AST）和丙氨酸氨基转移酶（ALT）的水平。通过 HE 染色观察肝脏和小肠的组织病理学变化。QRT-PCR 检测了肝组织中 miR-122 的表达水平。免疫印迹法检测肝组织中TLR4、MyD88、NF- κB p65和p-p65的蛋白表达：结果表明：HRG 可减缓非酒精性脂肪肝大鼠的体重增加，降低（PC）：结论：HRG可通过调节miR-122介导的TLR4/MyD88/NF-κB通路减轻非酒精性脂肪肝大鼠的肝脏和肠道损伤。

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来源期刊

Combinatorial chemistry & high throughput screening 化学-生化研究方法

CiteScore

3.10

自引率

5.60%

发文量

327

审稿时长

7.5 months

期刊介绍： Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.