Predictive Value of dsDNA and Nucleosomes as Neutrophil Extracellular Traps-Related Biomarkers for COVID-19 in Older Patients.

IF 4.1 2区医学 Q2 IMMUNOLOGY Journal of Inflammation Research Pub Date : 2024-11-15 eCollection Date: 2024-01-01 DOI:10.2147/JIR.S414688

Xudong Cui, Tiewei Li, Jingping Yang, Xiaojuan Li, Pengfei Xuan, Hongyan Wang

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Abstract

Background: Previous studies have demonstrated that neutrophil extracellular traps (NETs) are crucial in infectious diseases. This study aims to evaluate the clinical value of NET-related biomarkers in identifying the risk of COVID-19 and diagnosing the disease.

Methods: This study involved 32 patients who tested positive for COVID-19 via polymerase chain reaction (PCR) between April and August 2023. During the same period, 30 healthy volunteers were enrolled as a control group. The principal biomarkers related to NETs are citrullinated histone H3 (CitH3), double-stranded DNA (dsDNA), myeloperoxidase-DNA complex (MPO-DNA), and Nucleosome. Elevated levels in two or more of these biomarkers indicate raised NET concentrations. Multivariable logistic regression analysis was employed to assess whether NET-related biomarkers were the independent risk factor of COVID-19. The diagnostic value of NET-related biomarkers in COVID-19 was further evaluated using receiver operating characteristic (ROC) curve analysis. Statistical procedures were executed in SPSS software (version 24.0, USA).

Results: Compared with the control group, patients infected with COVID-19 had higher levels of dsDNA and nucleosomes (P < 0.001). Correlation analysis revealed a positive correlation between dsDNA levels and neutrophil count (r = 0.309, P = 0.015) as well as between nucleosome levels and neutrophil count (r = 0.446, P < 0.001). Further analysis showed that dsDNA and nucleosomes were independent risk factors for COVID-19 infection. ROC curve analysis showed that dsDNA area under the curve (AUC) = 0.777, 95% confidence interval (CI), 0.661-0.893, P < 0.001, and nucleosomes (AUC = 0.884, 95% CI, 0.778-0.991, P < 0.001) had well diagnostic value in the diagnosing COVID-19 infection.

Conclusion: NET-related biomarkers, dsDNA and nucleosomes, were independent risk factors of COVID-19 infection and potentially useful biomarkers in diagnosing COVID-19 infection in older patients.

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dsDNA和核小体作为中性粒细胞胞外陷阱相关生物标记物对老年患者COVID-19的预测价值

背景：先前的研究表明，中性粒细胞胞外捕获物（NET）在感染性疾病中至关重要。本研究旨在评估NET相关生物标志物在识别COVID-19风险和诊断疾病方面的临床价值：本研究涉及 2023 年 4 月至 8 月间通过聚合酶链反应（PCR）检测出 COVID-19 阳性的 32 名患者。同期还招募了 30 名健康志愿者作为对照组。与 NET 相关的主要生物标志物是瓜氨酸组蛋白 H3（CitH3）、双链 DNA（dsDNA）、髓过氧化物酶-DNA 复合物（MPO-DNA）和核糖体。其中两种或两种以上生物标志物的水平升高表明 NET 浓度升高。采用多变量逻辑回归分析来评估与NET相关的生物标志物是否是COVID-19的独立风险因素。利用接收器操作特征曲线（ROC）分析进一步评估了COVID-19中NET相关生物标志物的诊断价值。统计程序在SPSS软件（24.0版，美国）中执行：与对照组相比，COVID-19 感染者的dsDNA和核糖体水平更高（P < 0.001）。相关性分析表明，dsDNA水平与中性粒细胞计数呈正相关（r = 0.309，P = 0.015），核小体水平与中性粒细胞计数呈正相关（r = 0.446，P < 0.001）。进一步分析表明，dsDNA和核糖体是COVID-19感染的独立风险因素。ROC曲线分析显示，dsDNA曲线下面积（AUC）=0.777，95%置信区间（CI），0.661-0.893，P＜0.001，核糖体（AUC=0.884，95%CI，0.778-0.991，P＜0.001）对诊断COVID-19感染具有很好的诊断价值：与NET相关的生物标志物dsDNA和核糖体是COVID-19感染的独立危险因素，也是诊断老年患者COVID-19感染的潜在有用生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Inflammation Research Immunology and Microbiology-Immunology

CiteScore

6.10

自引率

2.20%

发文量

658

审稿时长

16 weeks

期刊介绍： An international, peer-reviewed, open access, online journal that welcomes laboratory and clinical findings on the molecular basis, cell biology and pharmacology of inflammation.