Aberrant frequency of circulating IL-21+ T follicular helper cells in patients with primary focal segmental glomerulosclerosis

IF 3.2 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular immunology Pub Date : 2024-11-19 DOI:10.1016/j.molimm.2024.11.001

Jing Liu , Yanbo Wang , Zhihui Qu , Junzhuo Si , Yanfang Jiang

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Abstract

Follicular helper T (Tfh) cells have been implicated in the pathophysiology of numerous diseases. This study investigated the hypothetical function of peripheral blood IL-21+ Tfh cells in the etiology of focal segmental glomerulosclerosis (FSGS). Tfh cell subsets were identified via flow cytometry in PBMCs from 15 patients with FSGS and 9 healthy controls (HCs). Moreover, a cytometric bead array (CBA) was used to determine the level of IL-21 in the serum. The proportions of IL-21+ cTfh cells, IL-21+ PD-1+ cTfh cells and serum IL-21 were lower in FSGS patients than in HCs. In FSGS patients, the serum IL-21 concentration was positively correlated with the frequency of IL-21+ cTfh cells and IL-21+ PD-1+ cTfh cells. The frequencies of IL-21+ cTfh cells and IL-21+ PD-1+ cTfh cells were negatively associated with 24-h urine protein but positively correlated with eGFR, serum albumin and serum IgG.

Conclusions

An aberrant frequency of IL-21+ Tfh cells was detected in FSGS patients, which may provide a better understanding of FSGS pathogenesis.

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原发性局灶节段性肾小球硬化症患者循环中IL-21+ T滤泡辅助细胞的异常频率。

滤泡辅助 T（Tfh）细胞与许多疾病的病理生理学有关。本研究探讨了外周血 IL-21+ Tfh 细胞在局灶节段性肾小球硬化症（FSGS）病因中的假设功能。通过流式细胞术鉴定了 15 名 FSGS 患者和 9 名健康对照者（HCs）的 PBMC 中的 Tfh 细胞亚群。此外，还使用细胞计数珠阵列（CBA）测定了血清中 IL-21 的水平。FSGS患者IL-21+ cTfh细胞、IL-21+ PD-1+ cTfh细胞和血清IL-21的比例均低于HCs。在 FSGS 患者中，血清 IL-21 浓度与 IL-21+ cTfh 细胞和 IL-21+ PD-1+ cTfh 细胞的频率呈正相关。IL-21+ cTfh 细胞和 IL-21+ PD-1+ cTfh 细胞的频率与 24 小时尿蛋白呈负相关，但与 eGFR、血清白蛋白和血清 IgG 呈正相关。结论：在FSGS患者中发现了IL-21+ Tfh细胞的异常频率，这可能有助于更好地了解FSGS的发病机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular immunology 医学-免疫学

CiteScore

6.90

自引率

2.80%

发文量

324

审稿时长

50 days

期刊介绍： Molecular Immunology publishes original articles, reviews and commentaries on all areas of immunology, with a particular focus on description of cellular, biochemical or genetic mechanisms underlying immunological phenomena. Studies on all model organisms, from invertebrates to humans, are suitable. Examples include, but are not restricted to: Infection, autoimmunity, transplantation, immunodeficiencies, inflammation and tumor immunology Mechanisms of induction, regulation and termination of innate and adaptive immunity Intercellular communication, cooperation and regulation Intracellular mechanisms of immunity (endocytosis, protein trafficking, pathogen recognition, antigen presentation, etc) Mechanisms of action of the cells and molecules of the immune system Structural analysis Development of the immune system Comparative immunology and evolution of the immune system "Omics" studies and bioinformatics Vaccines, biotechnology and therapeutic manipulation of the immune system (therapeutic antibodies, cytokines, cellular therapies, etc) Technical developments.