Anti-Inflammatory and Anti-proliferative Role of Essential Oil of Leaves of Cleistocalyx operculatus (Roxb.) Merr. & Perry.

IF 2.6 4区医学 Q3 CHEMISTRY, MEDICINAL Anti-cancer agents in medicinal chemistry Pub Date : 2024-11-20 DOI:10.2174/0118715206304193240715043704

Vivek Pandey, Sumnath Khanal, Nerina Shahi, Rupak Parajuli, Achyut Adhikari, Yuba Raj Pokharel

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Abstract

Background: Phytochemicals have long remained an essential component of the traditional medicine system worldwide. Advancement of research in phytochemicals has led to the identification of novel constituents and metabolites from phytochemicals, performing various vital functions ranging from antimicrobial properties to anticarcinogenic roles. This plant is traditionally used by local people to manage inflammation. In this study, we aim to extract and chemically profile the essential oil from the leaves of Cleistocalyx operculatus (Roxb.) Merr. & Perry and study of the anti-inflammatory and anti-proliferative role of essential oil.

Methods: The hydro distillation method was used for the extraction of essential oil, and the GC-MS was applied for the chemical profiling. The percentage of cell viability was calculated using a crystal violet assay, colony formation assay was performed using Semiquantitative PCR, Propodium iodite staining was used for cell death assay, and Western blotting was used to determine antibodies and proteins. Schrodinger 2015 software was used for molecular docking.

Results: Myrcene, a monoterpene, constitutes 56% of the oil and could be attributed to its anti-inflammatory potential. Treatment of LPS-challenged mouse macrophages RAW264.7 cells with essential oil resulted in a decline in the inflammatory markers, such as IL-1β, TNFα, iNOS, COX-2, and NFκB. Further, essential oil inhibited cancer PC-3, A431, A549, and MCF-7 cell lines at concentrations lower than normal PNT2 and HEK-293 cell lines. This decline in proliferative potential can be attributed to a decline in anti-apoptotic proteins, such as procaspase 3 and PARP, an increase in CKIs, such as p21, and a decline in the Akt signaling responsible for survival.

Conclusion: The essential oil of the plant Cleistocalyx operculatus may be a potential lead for anti-inflammatory and anti-proliferative function.

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Cleistocalyx operculatus (Roxb.) Merr. & Perry.

背景：长期以来，植物化学物一直是全球传统医学体系的重要组成部分。随着对植物化学物质研究的不断深入，人们从植物化学物质中发现了新的成分和代谢物，它们具有从抗菌到抗癌等多种重要功能。当地人传统上使用这种植物来控制炎症。本研究旨在从 Cleistocalyx operculatus (Roxb.) Merr. & Perry 的叶子中提取精油并对其进行化学分析，同时研究精油的抗炎和抗增殖作用：方法：采用水蒸馏法提取精油，并采用气相色谱-质谱仪进行化学分析。细胞存活率用水晶紫检测法计算，菌落形成检测用半定量 PCR 法进行，细胞死亡检测用碘化钠染色法，抗体和蛋白质检测用 Western 印迹法。使用 Schrodinger 2015 软件进行分子对接：蓖麻油中56%的成分为单萜烯，这可能是蓖麻油具有抗炎潜力的原因。用精油处理受到 LPS 挑战的小鼠巨噬细胞 RAW264.7 会导致炎症指标下降，如 IL-1β、TNFα、iNOS、COX-2 和 NFκB。此外，精油对 PC-3、A431、A549 和 MCF-7 癌细胞株的抑制浓度低于正常的 PNT2 和 HEK-293 细胞株。这种增殖潜力的下降可归因于抗凋亡蛋白（如促凋亡酶 3 和 PARP）的减少、CKIs（如 p21）的增加以及负责存活的 Akt 信号的下降：结论：Cleistocalyx operculatus 植物精油可能是抗炎和抗增殖功能的潜在线索。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anti-cancer agents in medicinal chemistry ONCOLOGY-CHEMISTRY, MEDICINAL

CiteScore

5.10

自引率

3.60%

发文量

323

审稿时长

4-8 weeks

期刊介绍： Formerly: Current Medicinal Chemistry - Anti-Cancer Agents. Anti-Cancer Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of anti-cancer agents. Each issue contains a series of timely in-depth reviews and guest edited issues written by leaders in the field covering a range of current topics in cancer medicinal chemistry. The journal only considers high quality research papers for publication. Anti-Cancer Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in cancer drug discovery.