The functional TNF-α^-308G > a single-nucleotide polymorphism (rs1800629): association with the predictive indices of breast cancer carcinogenesis.

IF 3 3区医学 Q2 ONCOLOGY Breast Cancer Research and Treatment Pub Date : 2024-11-21 DOI:10.1007/s10549-024-07536-y

Sherif Refaat, Hanan E Al-Rashidi, Rania A Abd El Azeem, Walaa E Nouh, Sahar Hamed, Zeinab R Attia

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Abstract

Background: Compared with all other cancer types, Breast cancer (BC) among women has now exceeded them all as the primary reason for cancer worldwide. The BC represents 11.7% of all cancer cases and accounts for a predestined 2.3 million new cases. It is the fourth primary reason for cancer-associated deaths in women. With a staggering 200-400% increase in the relative incidence of BC in Egypt, there is an urgent need for new diagnostic or predictive markers.

Purpose: The current investigation aims to explore the connection of the functional TNF-α^-308G > A (rs1800629) single-nucleotide polymorphism (SNP) with different breast cancer predictive indices.

Methods: The ARMS-PCR method was used for genotyping TNF-α^-308G > A SNP. Three groups were recruited for the study: 79 patients with benign breast inflammation (BBI); 163 with breast cancer (BC) and 144 controls (C).

Results: The TNF-α^-308G > A SNP was distributed among different groups in a unique pattern; in the control group 63.9% of cases were in the GG, 34% were in the GA, and 2.1% were in the AA. The BC group had 14% GG, 79% GA, and 7% AA, while the BBI group had 24% GG, 76% GA, and 0% AA. The AA genotype and A allele represented a strong significant correlation with risk factors in the BC group (OR_AA: 14.67 [95% CI = 3.78-56.91] and OR_A: 0.27 [95% CI = 0.19-0.39], respectively; P < 0.0001) in contrast to the control group. However, in the BBI group, a strong significant correlation was noted with the GA genotype (OR_GA: 5.93 [95% CI = 3.18-11.04] P < 0.0001). In the BC group, the AA genotype shows a significant increase in Nottingham Prognostic Index (NPI) in positive ER and PR in contrast to the relevant negative ones (P = 0.02 and 0.002, respectively). However, the GA genotype significantly increased NPI in positive Her2 and metastatic patients (P = 0.03 and 0.01, respectively).

Conclusion: This research is the first to correlate TNF-α^-308G > A (rs1800629) SNP in Egyptian BC patients. The A allele, GA & AA genotypes, and the Overdominant model of the TNF-α^-308G > A gene variants were recorded as prognostic risk factors for BC carcinogenesis.

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功能性 TNF-α-308G > a 单核苷酸多态性（rs1800629）：与乳腺癌癌变预测指标的关联。

背景：与所有其他癌症类型相比，女性乳腺癌（BC）目前已超过所有癌症类型，成为全球癌症的首要原因。乳腺癌占所有癌症病例的 11.7%，新增病例达 230 万例。它是妇女因癌症死亡的第四大主要原因。在埃及，乳腺癌的相对发病率惊人地增长了 200-400%，因此迫切需要新的诊断或预测标志物。目的：本次调查旨在探索功能性 TNF-α-308G > A (rs1800629) 单核苷酸多态性（SNP）与不同乳腺癌预测指数之间的联系：方法：采用ARMS-PCR方法对TNF-α-308G > A SNP进行基因分型。研究共招募了三组患者：79名良性乳腺炎症（BBI）患者、163名乳腺癌（BC）患者和144名对照组（C）：结果：TNF-α-308G > A SNP 在不同组别中的分布具有独特的模式；在对照组中，63.9%的病例为 GG 型，34%为 GA 型，2.1%为 AA 型。BC 组中 GG 占 14%，GA 占 79%，AA 占 7%，而 BBI 组中 GG 占 24%，GA 占 76%，AA 占 0%。在 BC 组中，AA 基因型和 A 等位基因与风险因素有很强的相关性（ORAA：14.67 [95% CI = 3.78-56.91] 和 ORA：0.27 [95% CI = 0.19-0.39] ；P GA：5.93 [95% CI = 3.18-11.04] P 结论：该研究首次将 TNF-α-308G > A (rs1800629) SNP 与埃及 BC 患者相关联。A等位基因、GA和AA基因型以及TNF-α-308G > A基因变异的超显性模型被记录为BC癌变的预后风险因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Breast Cancer Research and Treatment 医学-肿瘤学

CiteScore

6.80

自引率

2.60%

发文量

342

审稿时长

1 months

期刊介绍： Breast Cancer Research and Treatment provides the surgeon, radiotherapist, medical oncologist, endocrinologist, epidemiologist, immunologist or cell biologist investigating problems in breast cancer a single forum for communication. The journal creates a "market place" for breast cancer topics which cuts across all the usual lines of disciplines, providing a site for presenting pertinent investigations, and for discussing critical questions relevant to the entire field. It seeks to develop a new focus and new perspectives for all those concerned with breast cancer.