Enhancing vaginal reconstruction through 3D bioprinted scaffolds using a novel vECM-GelMA-SF bioink.

IF 8.2 2区 医学 Q1 ENGINEERING, BIOMEDICAL Biofabrication Pub Date : 2024-12-10 DOI:10.1088/1758-5090/ad95bf
Jiahua Zheng, Xuemei Zhang, Kaixuan Guo, Liman Yan, Xiaotong Xu, Wenxin Shi, Jingkun Zhang, Yanfang Du, Mingle Zhang, Xianghua Huang
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Abstract

Overcoming the low cell survival rates and insufficient neovascularization associated with tissue engineering of the vagina is crucial for advancing the vaginal reconstruction. In this research, we have developed a unique bioink composed of porcine vaginal extracellular matrix (vECM), gelatin methacrylamide (GelMA), and silk fibroin (SF) to facilitate the bioprinting of a vaginal scaffold. The vECM-GelMA-SF bioink effectively replicates thein vivomicroenvironment, supporting thein vitrocultivation of 3D bioprinted vaginal scaffolds. It promotes stem cell viability and enhances neovascularization by harnessing the mechanical properties of GelMA/SF and the tissue specificity of vECM.In vivoorthotopic studies have demonstrated that the use of 3D bioprinted vaginal scaffolds significantly improves the functionality of reconstructed vaginas, promoting angiogenesis, rapid epithelialization, muscle regeneration, glycogen secretion, and nerve repair. The reconstructed vaginal tissues in the 3D cell-loaded scaffold group closely resemble natural vaginal tissues. Differential proteomics analysis has provided insights into the genetic functions and biological pathways involved in vaginal reconstruction. Our study successfully optimized the composition of the vECM-GelMA-SF bioink, achieving a balance between biocompatibility and printability. This bioink is suitable for constructing 3D bioprinted vaginal scaffolds of various dimensions, transplantablein situin animal models with different degrees of vaginal absence. The bioink may find applications in clinical settings, improving the overall effectiveness and safety ofin vivovaginal reconstruction procedures.

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使用新型 vECM-GelMA-SF 生物墨水,通过三维生物打印支架加强阴道重建。
克服与阴道组织工程相关的细胞存活率低和新生血管不足的问题对于推进阴道重建至关重要。在这项研究中,我们开发了一种由猪阴道细胞外基质(vECM)、明胶甲基丙烯酰胺(Gelatin Methacrylamide,GelMA)和丝纤维素(SF)组成的独特生物墨水,以促进阴道支架的生物打印。vECM-GelMA-SF 生物墨水能有效复制体内微环境,支持三维生物打印阴道支架的体外培养。它利用 GelMA/SF 的机械特性和 vECM 的组织特异性,提高了干细胞的活力,增强了血管新生。活体移植研究表明,使用三维生物打印阴道支架能显著改善重建阴道的功能,促进血管生成、快速上皮化、肌肉再生、糖原分泌和神经修复。三维细胞负载支架组重建的阴道组织与天然阴道组织非常相似。差异蛋白质组学分析深入揭示了阴道重建所涉及的遗传功能和生物通路。我们的研究成功地优化了 vECM-GelMA-SF 生物墨水的成分,实现了生物相容性和可打印性之间的平衡。这种生物墨水适用于构建各种尺寸的三维生物打印阴道支架,可在不同阴道缺失程度的动物模型中进行原位移植。这种生物墨水可应用于临床,提高体内阴道重建手术的整体有效性和安全性。
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来源期刊
Biofabrication
Biofabrication ENGINEERING, BIOMEDICAL-MATERIALS SCIENCE, BIOMATERIALS
CiteScore
17.40
自引率
3.30%
发文量
118
审稿时长
2 months
期刊介绍: Biofabrication is dedicated to advancing cutting-edge research on the utilization of cells, proteins, biological materials, and biomaterials as fundamental components for the construction of biological systems and/or therapeutic products. Additionally, it proudly serves as the official journal of the International Society for Biofabrication (ISBF).
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