Gyutae Park, Sieun S Kim, Jiwon Shim, Seung-Jae V Lee
{"title":"Brief guide to immunostaining.","authors":"Gyutae Park, Sieun S Kim, Jiwon Shim, Seung-Jae V Lee","doi":"10.1016/j.mocell.2024.100157","DOIUrl":null,"url":null,"abstract":"<p><p>Immunostaining is an essential biological technique that determines the localization and level of target antigen molecules, using antibodies within cells or tissues. Here we present a brief guide to immunostaining, including the principles, methods and different types of immunostaining. This manuscript will also provide common challenges and optimization strategies. This work will be useful for researchers with basic knowledge in immunostaining.</p>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":" ","pages":"100157"},"PeriodicalIF":3.7000,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecules and Cells","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.mocell.2024.100157","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Immunostaining is an essential biological technique that determines the localization and level of target antigen molecules, using antibodies within cells or tissues. Here we present a brief guide to immunostaining, including the principles, methods and different types of immunostaining. This manuscript will also provide common challenges and optimization strategies. This work will be useful for researchers with basic knowledge in immunostaining.
期刊介绍：
Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is ''Mol. Cells''. Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
