Effects of blood donor characteristics and storage on red blood cell haemoglobin β S-nitrosylation.

IF 1.8 4区医学 Q3 HEMATOLOGY Vox Sanguinis Pub Date : 2024-11-21 DOI:10.1111/vox.13768

Cuiping Zhang, Peng Huang, Ravinder J Singh, Abba C Zubair

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Abstract

Background and objectives: In the setting of tissue hypoxia, S-nitrosylated haemoglobin (SNO-Hb) plays crucial roles in the control of blood flow. This is associated with decreased oxygen affinity to haemoglobin and increase in tissue oxygenation. Red blood cell (RBC) transfusion is primarily performed to improve tissue oxygenation in anaemic patients. RBCs after storage undergo a variety of biochemical and functional alterations, including deficiency of nitric oxide (NO) bioactivity. However, how donor characteristics affect NO levels during RBC storage is unclear. We sought to investigate the association of blood donor age, gender and storage duration with NO and SNO-Hb levels in blood units.

Materials and methods: Blood samples from 42 healthy younger (≤30 years) and older (≥45 years) donors were collected and stored for up to 42 days. Total NO kits were used to detect total nitrite and nitrate levels in blood storage solution. SNO-Hb levels in RBCs were detected and analysed by quantitative mass spectrometry.

Results: Total NO levels in the blood storage solution significantly increased with donor age and storage duration. Proteomic analysis revealed that RBCs from older donors, particularly older females, significantly lost SNO-Hb during storage. Our findings indicate that RBCs from older donors are associated with reduced SNO-Hb levels and increased NO metabolites in storage solution after ≥35 days storage.

Conclusion: The findings suggest stored RBCs from older donors may have reduced capacity to deliver oxygen to tissues under hypoxia. A shorter shelf life may be required for storing RBCs from older donors, particularly older females.

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献血者特征和储存对红细胞血红蛋白 β S-亚硝基化的影响

背景和目的：在组织缺氧的情况下，S-亚硝基化血红蛋白（SNO-Hb）在控制血流量方面发挥着至关重要的作用。这与血红蛋白的氧亲和力下降和组织氧合增加有关。输注红细胞主要是为了改善贫血患者的组织氧合。储存后的红细胞会发生各种生化和功能变化，包括一氧化氮（NO）生物活性的缺乏。然而，目前还不清楚供体特征如何影响 RBC 储存过程中的一氧化氮水平。我们试图研究献血者年龄、性别和储存时间与血单位中一氧化氮和 SNO-Hb 水平的关系：收集了 42 名年轻（≤30 岁）和年长（≥45 岁）的健康献血者的血样，并将其储存长达 42 天。使用总 NO 试剂盒检测血液储存液中的总亚硝酸盐和硝酸盐水平。用定量质谱法检测和分析红细胞中的 SNO-Hb 含量：结果：血液储存液中的总氮氧化物水平随供体年龄和储存时间的延长而显著增加。蛋白质组分析表明，年龄较大的献血者，尤其是老年女性的红细胞在储存过程中明显丢失 SNO-Hb。我们的研究结果表明，年龄较大的献血者的红细胞在储存≥35 天后，储存液中的 SNO-Hb 水平降低，NO 代谢物增加：结论：研究结果表明，来自年长供体的储存红细胞在缺氧情况下向组织输送氧气的能力可能会降低。结论：研究结果表明，年龄较大的供体储存的红细胞在缺氧情况下向组织输送氧气的能力可能会降低，因此需要缩短年龄较大供体的红细胞（尤其是老年女性）的储存期限。

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来源期刊

Vox Sanguinis 医学-血液学

CiteScore

4.40

自引率

11.10%

发文量

156

审稿时长

6-12 weeks

期刊介绍： Vox Sanguinis reports on important, novel developments in transfusion medicine. Original papers, reviews and international fora are published on all aspects of blood transfusion and tissue transplantation, comprising five main sections: 1) Transfusion - Transmitted Disease and its Prevention: Identification and epidemiology of infectious agents transmissible by blood; Bacterial contamination of blood components; Donor recruitment and selection methods; Pathogen inactivation. 2) Blood Component Collection and Production: Blood collection methods and devices (including apheresis); Plasma fractionation techniques and plasma derivatives; Preparation of labile blood components; Inventory management; Hematopoietic progenitor cell collection and storage; Collection and storage of tissues; Quality management and good manufacturing practice; Automation and information technology. 3) Transfusion Medicine and New Therapies: Transfusion thresholds and audits; Haemovigilance; Clinical trials regarding appropriate haemotherapy; Non-infectious adverse affects of transfusion; Therapeutic apheresis; Support of transplant patients; Gene therapy and immunotherapy. 4) Immunohaematology and Immunogenetics: Autoimmunity in haematology; Alloimmunity of blood; Pre-transfusion testing; Immunodiagnostics; Immunobiology; Complement in immunohaematology; Blood typing reagents; Genetic markers of blood cells and serum proteins: polymorphisms and function; Genetic markers and disease; Parentage testing and forensic immunohaematology. 5) Cellular Therapy: Cell-based therapies; Stem cell sources; Stem cell processing and storage; Stem cell products; Stem cell plasticity; Regenerative medicine with cells; Cellular immunotherapy; Molecular therapy; Gene therapy.