Whole-Genome sequencing of Calonectria dianii: An important pathogen causing Eucalyptus leaf blight

Abstract

Eucalyptus leaf blight, caused by Calonectria spp., significantly impacts the global Eucalyptus industry. Calonectria dianii, as one of the predominant causal agents, poses a serious threat to Eucalyptus plantations in China. To enhance our understanding of its pathogenic mechanisms, we sequenced the genome of C. dianii RIFT 6520 using both Nanopore PromethION and Illumina NovaSeq PE150 platforms. Our analysis revealed a 61.76 Mb genome comprising 30 contigs with an N50 of 4,726,631 bp, a GC content of 49.74 %, and 10,184 predicted coding genes. Additionally, comparative genomic analysis between C. dianii and seven other significant plant-pathogenic Calonectria species was conducted. This analysis provided insights into the evolutionary relationships and adaptive mechanisms of these pathogens. Our study elucidates the genetic basis of C. dianii's pathogenicity and evolution, providing valuable information for future research on its molecular interactions with Eucalyptus and aiding in the development of precise control measures for Eucalyptus leaf blight.