Influence of Influent Load on Nitrification/Denitrification with MBBR for Oil Shale Retorting Wastewater Treatment: Performance and Microbial Community Structure

IF 3.8 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES Water, Air, & Soil Pollution Pub Date : 2024-11-25 DOI:10.1007/s11270-024-07615-2

Rong-xin Zhang, Yi Liu, Dian-nan Huang, Li Zhang, Xing-guan Ma, Peng-fei Yu, Chuang Liu, Yong-yong Wang

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Abstract

Oil shale retorting wastewater is currently recognized as one of the most complex industrial wastewater due to its ultra-low chemical oxygen demand to total nitrogen (C/N) ratio, high organic load, and complex chemical composition. In this paper, the effect of influent load on the performance and microbial community changes of moving bed biofilm reactor(MBBR) in the treatment of highly polluted retorting wastewater was investigated. The MBBR was proposed and demonstrated to be efficient in treating the retorting wastewater, containing COD and NH₄⁺-N of 5000-10000mg/L and 3000-5000mg/L. The removal efficiency of COD and NH₄⁺-N increased steadily in 1-79d, and the effect was the best when the influent load was 0.0242–0.0312kg COD/kg MLSS·d and 0.0173–0.0208kg NH₄⁺-N/kg MLSS·d. The removal efficiency of COD was up to 83.46%, and the removal efficiency of NH₄⁺-N was 82.08%. The long-term operation showed that the biofilm could be rapidly formed after 40 days. Scanning electron microscope observation showed that cocci and short bacilli appeared in the pores of the fillers. With the increase of influent load, the total amount of extracellular polymeric substances increased significantly, in which the content of protein increased sharply in stage III, while the polysaccharide was in a state of continuous and stable growth. High-throughput sequencing analysis showed that the nitrifying functional genus Nitrosomonas was highly enriched in the MBBR system (0 → 17.55%) and played a key role in the high accumulation of NO₃⁻-N. The newly added denitrifying bacteria Brevundimonas (5.85%) and Reyranella (4.57%) enhanced the degradation of COD and maintained the nitrogen cycle in the system. The enrichment of Acinetobacter, Rugosibacter, and unclassified_Syntrophobacteraceae in the system played an important role in the degradation of complex organic compounds such as polycyclic aromatic hydrocarbons and aromatic compounds.

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油页岩蒸馏废水处理 MBBR 的进水负荷对硝化/反硝化的影响：性能和微生物群落结构

油页岩蒸馏废水因其超低的化学需氧量与总氮（C/N）比、高有机负荷和复杂的化学成分而被认为是目前最复杂的工业废水之一。本文研究了进水负荷对移动床生物膜反应器（MBBR）处理高污染蒸馏废水的性能和微生物群落变化的影响。结果表明，移动床生物膜反应器能有效处理含 COD 和 NH4+-N 分别为 5000-10000mg/L 和 3000-5000mg/L 的蒸馏废水。COD 和 NH4+-N 的去除率在 1-79d 内稳步上升，当进水负荷为 0.0242-0.0312kg COD/kg MLSS-d 和 0.0173-0.0208kg NH4+-N/kg MLSS-d 时效果最好。COD 的去除率高达 83.46%，NH4+-N 的去除率为 82.08%。长期运行表明，40 天后生物膜可迅速形成。扫描电镜观察表明，填料孔隙中出现了球菌和短杆菌。随着进水负荷的增加，细胞外高分子物质总量显著增加，其中蛋白质含量在第三阶段急剧增加，而多糖则处于持续稳定增长状态。高通量测序分析表明，硝化功能菌属 Nitrosomonas 在 MBBR 系统中高度富集（0 → 17.55%），在 NO3-N 的高积累过程中发挥了关键作用。新加入的反硝化细菌 Brevundimonas（5.85%）和 Reyranella（4.57%）增强了 COD 的降解，维持了系统中的氮循环。系统中富集的不动杆菌（Acinetobacter）、鲁戈西杆菌（Rugosibacter）和未分类的合成分支杆菌科（Syntrophobacteraceae）在降解多环芳烃和芳香族化合物等复杂有机化合物方面发挥了重要作用。

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来源期刊

Water, Air, & Soil Pollution 环境科学-环境科学

CiteScore

4.50

自引率

6.90%

发文量

448

审稿时长

2.6 months

期刊介绍： Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments. Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation. Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.