Simultaneous Quantitation of Bioactive Compounds in Different Varieties of Arachis Hypogea L. Using Liquid Chromatography/ESI Mass Spectrometry: A Chemometric Approach

IF 1.2 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS Chromatographia Pub Date : 2024-09-30 DOI:10.1007/s10337-024-04368-2

Shiv Nandan, Mohd Amish Khan, Mohsin Ali Khan, Vijaya Shukla, Madhumita Srivastava, Mohammad Faheem Khan

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Abstract

Cultivated Arachis hypogaea L., is commonly known as peanut. Its seed coat, also referred to as a shell, plays a critical role in safeguarding the seeds and facilitating the transportation of diverse metabolites. In the current work, an examination of metabolite profiling was executed utilizing UPLC-ESI–MS/MS in the seed coat (SeC), seed skin (SeS), and seed pulp (SeP) of four distinct peanut varieties of different geographical locations in India. The method was also validated according to ICH guidelines. A linear detector response was observed within a concentration range of 0.019–20.0 ng/mL, R² = 0.999. The limit of detection (LOD) and quantification (LOQ) of the targeted compounds were found to be within the range of 0.075–0.426 and 0.227–1.292 ng/mL, respectively. The intra-day and inter-day precision demonstrated relative standard deviation %RSD < 2%. The results of Principal Component Analysis (PCA) have demonstrated that the differentiation between varieties of peanut is attributable to the existence of luteolin and rutin having a greater impact on the identification of varieties.

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使用液相色谱/ESI质谱法同时定量测定不同品种的蚕豆中的生物活性化合物：化学计量学方法

栽培花生（Arachis hypogaea L.）俗称花生。它的种皮也被称为外壳，在保护种子和促进各种代谢物的运输方面起着至关重要的作用。在目前的研究中，利用 UPLC-ESI-MS/MS 对印度不同地理位置的四个不同花生品种的种皮（SeC）、种皮（SeS）和种髓（SeP）进行了代谢物分析。该方法还根据 ICH 指南进行了验证。在 0.019-20.0 纳克/毫升的浓度范围内，检测器呈线性响应，R2 = 0.999。目标化合物的检测限（LOD）和定量限（LOQ）分别在 0.075-0.426 和 0.227-1.292 纳克/毫升范围内。日内和日间精密度的相对标准偏差为 2%。主成分分析（PCA）的结果表明，花生品种之间的区分是由于叶黄素和芦丁的存在对品种的识别有较大的影响。

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.