A Validated RP-HPLC Method for Simultaneous Quantitative Determination of Lupeol, β-Amyrin, and Stigmasterol in Maytenus emarginata (Willd.) Ding Hou

IF 1.2 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS Chromatographia Pub Date : 2024-09-20 DOI:10.1007/s10337-024-04365-5

Supriya Sharma, Mahaveer Dhobi

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Abstract

The study aims at developing and validating a RP-HPLC (reverse phase high-performance liquid chromatography) method for the standardization of Maytenus emarginata (Willd.) Ding Hou extract by selecting Lupeol, β-Amyrin, and Stigmasterol as marker compounds. The developed analytical method used a Phenomenex C₈ column (250 × 4.6 mm; 5 μm) and the mobile phase of acetonitrile and HPLC (high-performance liquid chromatography) grade water (85:15) in isocratic elution at the flow rate 2.0 ml/min. Further, the compounds were traced at 202 nm with the run time of 9 min. The calibration curves presented good linear regression (R² > 0.999) in the tested concentrations. The developed method represented good repeatability for the estimation of the three compounds, namely Lupeol (0.009%), β-Amyrin (0.010%), and Stigmasterol (0.011%) in the samples having the RSD (relative standard deviation) of < 2% respectively. For all analytes, percentage recovery surpassed 90%. The validated RP-HPLC Method was utilized to quantify Lupeol, β-Amyrin, and Stigmasterol from methanolic extract of aerial parts of Maytenus emarginata (Willd.) Ding Hou.

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丁后马钱子（Maytenus emarginata (Willd.) Ding Hou）中羽扇豆醇（Lupeol）、β-香树脂醇（β-Amyrin）和豆甾醇（Stigmasterol）的 RP-HPLC 同时定量测定方法的验证

本研究以丁香提取物中的露贝醇、β-香树脂醇和豆固醇为标记物，建立并验证了丁香提取物的RP-HPLC（反相高效液相色谱）标准化分析方法。所建立的分析方法采用 Phenomenex C8 色谱柱（250 × 4.6 mm; 5 μm），以乙腈和 HPLC（高效液相色谱）级水（85:15）为流动相进行等度洗脱，流速为 2.0 ml/min。此外，在 202 纳米波长下对化合物进行检测，运行时间为 9 分钟。在测试浓度下，校准曲线呈现良好的线性回归（R2 > 0.999）。所建立的方法在估计样品中的三种化合物，即羽扇豆醇（0.009%）、β-杨梅素（0.010%）和豆甾醇（0.011%）时具有良好的重复性，RSD（相对标准偏差）分别为 2%。所有分析物的回收率均超过 90%。采用该方法定量检测了丁后马钱子气生部分甲醇提取物中的羽扇豆醇、β-香树脂醇和豆固醇。

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.