Improvement of lipid production from glucose/xylose mixed-sugar by the oleaginous yeast Lipomyces starkeyi through ultra-violet mutagenesis

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Enzyme and Microbial Technology Pub Date : 2024-11-22 DOI:10.1016/j.enzmictec.2024.110551

Sota Kamba, Ryosuke Yamada , Takuya Matsumoto, Hiroyasu Ogino

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Abstract

The oleaginous yeast Lipomyces starkeyi is a promising triacylglycerol (TAG) producer for biodiesel fuel. However, it is necessary to further improve TAG productivity in L. starkeyi from a mixed sugar of glucose and xylose. This study aimed to construct an L. starkeyi mutant with increased TAG productivity from glucose/xylose mixed-sugar and to elucidate the causes underlying increased lipid productivity. Ultra-violet (UV) mutagenesis combined with enrichment culture with ethanol and H₂O₂ and selection of low-density cells was applied to L. starkeyi to obtain the L. starkeyi mutant strain UMP47, which exhibited higher TAG production from glucose/xylose. Transcriptome analysis revealed high expression of genes involved in transporter activity and carbohydrate metabolism, whereas genes involved in DNA replication exhibited lower expression in the mutant strain UMP47 than in the wild-type strain. Altogether, the lipid productivity of L. starkeyi was successfully improved by UV mutagenesis. Transcriptome analysis suggested the importance of previously unidentified genes in TAG production. This study provides information on potential target genes for improving TAG production through the genetic modification of oleaginous yeast.

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通过紫外线诱变提高含油酵母星形脂酵母从葡萄糖/木糖混合糖中产生脂质的能力

含油酵母星形脂酵母是一种很有前途的生物柴油燃料三酰甘油（TAG）生产者。然而，有必要进一步提高星状酵母从葡萄糖和木糖混合糖中生产三酰甘油（TAG）的能力。本研究旨在构建一种可提高葡萄糖/木糖混合糖 TAG 生产率的星菌突变体，并阐明提高脂质生产率的原因。通过紫外线（UV）诱变、乙醇和 H2O2 富集培养以及低密度细胞的筛选，我们获得了星形菌突变株 UMP47，该突变株从葡萄糖/木糖中获得了更高的 TAG 产量。转录组分析显示，涉及转运体活性和碳水化合物代谢的基因表达量较高，而涉及 DNA 复制的基因在突变株 UMP47 中的表达量低于野生型菌株。总之，紫外诱变成功地提高了L. starkeyi的脂质生产率。转录组分析表明，以前未发现的基因在 TAG 生产中起着重要作用。这项研究为通过对含油酵母进行基因改造来提高 TAG 产量提供了潜在目标基因的信息。

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.