Association of ABCB1(Rs10276036, C/T) Gene, IL-18, and TNFα as Risk Factors for Nephrotic Syndrome Incidence.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2024-04-01 DOI:10.61186/rbmb.13.1.67

Eglal Aly Hassan, Afaf Mohamed Elsaid, Ahmed Mahmoud El-Refaey, Mohammed Abou Elzahab, Magdy Mahfouz Youssef, Rehab Elmougy

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Abstract

Background: The most common cause of Nephrotic Syndrome (NS) in children is idiopathic NS, also called nephrosis. The most prominent clinical signs are hyperlipidemia, severe proteinuria, edema, swelling of body tissues, and an increased risk of infection. The object of this study was to examine the correlation of the ABCB1 gene (rs10276036, C > T), IL-18, and TNFα to the prevalence of NS among Egyptian children having NS.

Methods: This study included 100 participants with NS and 100 healthy controls. To analyze the ABCB1 gene (rs10276036 C >T) variant PCR technique was used. IL-18 and TNF levels were estimated using Enzyme-Linked Immunosorbent Assay (ELISA).

Results: Increased frequency of CT and TT genotypes of the ABCB1 gene (rs10276036 C / T) in NS patients compared to controls, with p-value = 0.001, OR = 2.270, CI = (1.550-3.327) for CT genotype and p-value = 0.001, OR = 5.070, CI = (2.463-10.438) for TT genotype. The frequencies of ABCB1 (rs10276036 C >T) genotypes were statistically significant in the dominant model (OR 2.560; p< 0.001) and in the recessive model OR, 3.231; p= 0.001). Significantly high levels of both IL-18 and TNFα were found in NS patients compared to controls.

Conclusions: The ABCB1gene (rs10276036 C/T), IL-18, and TNFα are associated with the prevalence of NS in Egyptian children and might be considered as independent risk factors for its incidence.

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ABCB1（Rs10276036，C/T）基因、IL-18 和 TNFα 与肾病综合征发病率风险因素的关系

背景：儿童肾病综合征（NS）最常见的病因是特发性 NS，也称为肾病。最突出的临床表现是高脂血症、严重蛋白尿、水肿、身体组织肿胀和感染风险增加。本研究旨在探讨 ABCB1 基因（rs10276036，C > T）、IL-18 和 TNFα 与埃及 NS 儿童中 NS 患病率的相关性：本研究包括 100 名 NS 患者和 100 名健康对照者。采用 PCR 技术分析 ABCB1 基因（rs10276036 C >T）变异。使用酶联免疫吸附试验（ELISA）估测 IL-18 和 TNF 水平：与对照组相比，NS 患者 ABCB1 基因（rs10276036 C / T）CT 和 TT 基因型的频率增加，CT 基因型的 p 值 = 0.001，OR = 2.270，CI = (1.550-3.327)；TT 基因型的 p 值 = 0.001，OR = 5.070，CI = (2.463-10.438)。ABCB1（rs10276036 C >T）基因型的频率在显性模型（OR 2.560；p< 0.001）和隐性模型（OR 3.231；p= 0.001）中均具有统计学意义。与对照组相比，NS 患者的 IL-18 和 TNFα 水平明显偏高：ABCB1基因（rs10276036 C/T）、IL-18和TNFα与埃及儿童的NS发病率有关，可被视为NS发病率的独立风险因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

2.80

自引率

23.50%

发文量

审稿时长

10 weeks

期刊介绍： The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.