Image-based fuzzy logic control for pressure-driven droplet microfluidics as autosampler for multimodal imaging microscopy.

IF 6.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS Lab on a Chip Pub Date : 2024-11-26 DOI:10.1039/d4lc00583j

Fabian Ott, Tobias Meyer-Zedler, Michael Schmitt, Jürgen Popp

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Abstract

Here we present a highly customisable image-based fuzzy logic control (FLC) method for pressure-driven droplet microfluidics. The system is designed to position droplets of different sizes in microfluidic chips of varying channel size in the centre of the region of interest (ROI) using two parallel multiple input single output (MISO) FLCs. Overall, 95.1% of the droplets with an average displacement of 2.5 μm could be kept within the ROI during the pre-defined time intervals of up to 10 s. This is achieved by pre-determined pressure values that are kept constant during this time. The control principle was tested on different pressure controllers and microfluidic chips varying in material, channel layout and cross section. Droplet volumes ranged from a few hundred picolitres to a tenth of a microlitre. The droplets were composed of deionised water or contained two concentrations of S. cerevisiae. The average processing time was 12.5 seconds. This makes the method suitable for studying several hundred pre-sorted droplets from high-throughput screening (HTS) experiments.

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基于图像的模糊逻辑控制，用于压力驱动液滴微流体作为多模态成像显微镜的自动进样器。

在此，我们介绍一种高度可定制的基于图像的模糊逻辑控制（FLC）方法，用于压力驱动液滴微流控。该系统利用两个并行的多输入单输出（MISO）FLC，将不同大小的液滴定位在不同通道尺寸的微流控芯片中的感兴趣区（ROI）中心。总体而言，在长达 10 秒的预定时间间隔内，平均位移为 2.5 μm 的液滴中有 95.1% 能保持在 ROI 内。该控制原理在不同的压力控制器和微流控芯片上进行了测试，芯片的材料、通道布局和横截面各不相同。液滴体积从几百皮升到十分之一微升不等。液滴由去离子水组成，或含有两种浓度的麦角菌。平均处理时间为 12.5 秒。这使得该方法适用于研究来自高通量筛选（HTS）实验的数百个预分选液滴。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lab on a Chip 工程技术-化学综合

CiteScore

11.10

自引率

8.20%

发文量

434

审稿时长

2.6 months

期刊介绍： Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.