Rapid Identification of Chemical Constituents in New Compound Aloe Capsule Based on UPLC-Q-Orbitrap-MS

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS Rapid Communications in Mass Spectrometry Pub Date : 2024-11-26 DOI:10.1002/rcm.9944

Siyue Chen, Yajie Dou, Shumin Zhang, Huixin Qiu, Huiru Liu, Yanyan Xu, Lexin Shu, Yubo Li

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Abstract

Rationale

The chemical composition of the new compound aloe capsule (NCAC) has not been fully elucidated to date, which poses challenges for pharmacology, scientific, and rational drug use. In this study, a rapid and sensitive method was established to comprehensively study the constituents in the NCAC. The contents of the NCAC were analyzed by UPLC-Q-Orbitrap-MS and data postprocessing technology.

Methods

Firstly, the extract was separated by UPLC system, and the fragment information was obtained under positive and negative ions and then compared with the characteristic fragments described in the literature, so as to achieve the purpose of rapid identification of the compound of NCAC.

Results

A total of 56 compounds including 21 chromones, 6 pyranones, 7 anthraquinones, 7 anthrone, 5 alkaloids, 4 amino acids, and 6 other components were detected in NCAC by comparing the retention time and mass spectrometry information and retrieving the reference literature.

Conclusions

The UPLC-Q-Orbitrap-MS method was developed and utilized successfully to identify the major constituents in NCAC and would be helpful for improving the quality control standard of NCAC.

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基于 UPLC-Q-Orbitrap-MS 快速鉴定新型复方芦荟胶囊中的化学成分

理由：迄今为止，新型复方芦荟胶囊（NCAC）的化学成分尚未完全阐明，这给药理学、科学和合理用药带来了挑战。本研究建立了一种快速、灵敏的方法来全面研究新复方芦荟胶囊中的成分。采用 UPLC-Q-Orbitrap-MS 和数据后处理技术分析了国家农作物基因库中的成分：首先用UPLC系统对提取物进行分离，获得正负离子下的碎片信息，然后与文献中描述的特征碎片进行比对，从而达到快速鉴定国药准字号中化合物的目的：结果：通过比较保留时间和质谱信息，并检索参考文献，共检测出NCAC中的56种化合物，包括21种色酮类化合物、6种吡喃酮类化合物、7种蒽醌类化合物、7种蒽酮类化合物、5种生物碱类化合物、4种氨基酸类化合物和6种其他成分：UPLC-Q-Orbitrap-MS方法的开发和应用成功地鉴定了北沙参中的主要成分，有助于提高北沙参的质量控制标准。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.