Phylogenetic Characterization of Vibrio vulnificus Strains from Oysters and its Comparison with Clinical vcgE Genotype Strain.

Abstract

Twenty-one environmental Vibrio vulnificus strains from the Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE) bacteria collection, 20 of them isolated from oyster samples and 1 from a reported clinical case, were sequenced to analyze the genomic divergence between 2 genotypes, E-genotype and C-genotype, proposed by various groups to distinguish clinical (C) from environmental (E) V. vulnificus strains. As indicated in previous analyses of PFGE, MLST, and rtxA, 9 of the CICESE isolates were identified as vcgE, compared with 12 as vcgC. Separation of the genotypes into these 2 groups was confirmed in this study, based on the presence of certain genes in the 21 genomes, the presence of virulence factors, and rtxA sequencing. Most genomes from oyster isolates expressed rtxA-C type, with the exception being rtxA-M type detected in CICESE-594 a vcgE strain isolated from a clinical case. Although several genetic approaches clearly indicate differences between the C- and E-genotypes, none of them, including those in this study, can highlight a single factor that could be used to indicate the potential pathogenicity of V. vulnificus isolated from oysters.