Evaluation of Skin Color Supervision Genes in Chickpea Seeds by Multiomics.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biotechnology Pub Date : 2024-11-26 DOI:10.1007/s12033-024-01304-5

Yanming Ma, Lin Xu, Hongmei Zhuang, Faten A Abd-Eldaim, Zhonghua Tang, Youssef Dewer, Hao Wang

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Abstract

Seed samples of two types of chickpea (Cicer arietinum Linn.), including variety A (NRCGR-4452) and variety B (local varieties), with different seed colors, were collected every five days for a total of four times during the seed development period. Non-targeted metabolome and transcriptome sequencing were conducted to identify differentially expressed genes and metabolites associated with chickpea seed coat color. The results indicated that the relative quercetin, pelargonidin, luteolin, rutin, myricetin, kaempferol, glycitin, and naringin contents were higher in variety A than in variety B, and that carbohydrate and amino acid metabolites had a greater impact on flavonoid metabolites. Quercetin, luteolin, and kaempferol were most significantly associated with seed color differences, the associated enzyme genes were LOC101491583 (callose synthase 5-like), LOC101503703 (flavonoid 3',5'-hydroxylase), LOC101514158 (callose synthase 5), LOC101497872 (UDP-glycosyltransferase 74F1-like), LOC101500232 (callose synthase 7 isoform X1), LOC101511206 (UDP-glycosyltransferase 73C3-like), LOC101502065 (galactoside 2-alpha-L-fucosyltransferase), LOC101492791 (sulfoquinovosyl transferase SQD2), and LOC101509377 (flavonol synthase). Additionally, the gene transcription factor MYB44 may regulate UDP-glycosyltransferase 73C3 to affect seed color differences.

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通过多组学评估鹰嘴豆种子中的肤色监督基因

在种子发育期间，每五天收集一次两种鹰嘴豆（Cicer arietinum Linn.）的种子样本，包括品种 A（NRCGR-4452）和品种 B（当地品种），它们的种子颜色各不相同，共收集了四次种子样本。进行了非靶向代谢组和转录组测序，以确定与鹰嘴豆种皮颜色相关的差异表达基因和代谢产物。结果表明，品种 A 的相对槲皮素、木犀草素、叶黄素、芦丁、杨梅素、山奈酚、甘草亭和柚皮苷含量高于品种 B，碳水化合物和氨基酸代谢物对类黄酮代谢物的影响更大。槲皮素、木犀草素和山奈酚与种子颜色差异的关系最为显著，相关的酶基因有 LOC101491583（胼胝质合成酶 5-like）、LOC101503703（黄酮类化合物 3',5'-羟化酶）、LOC101514158（胼胝质合成酶 5）、LOC101497872（UDP-糖基转移酶 74F1-like）、LOC101500232（胼胝质合成酶 7 异构体 X1）、LOC101511206（UDP-糖基转移酶 73C3-like）、LOC101502065（半乳糖苷 2-α-L-岩藻糖基转移酶）、LOC101492791（磺基喹诺酮转移酶 SQD2）和 LOC101509377（黄酮醇合成酶）。此外，基因转录因子 MYB44 可能会调控 UDP-糖基转移酶 73C3 以影响种子颜色的差异。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.