Exploring the molecular interactions between nephrolithiasis and carotid atherosclerosis: asporin as a potential biomarker.

IF 2 2区医学 Q2 UROLOGY & NEPHROLOGY Urolithiasis Pub Date : 2024-11-26 DOI:10.1007/s00240-024-01665-1

Yibo Hua, Zijian Zhou, Suyu Miao, Zijie Wang, Rijin Song, Xianghu Meng

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Abstract

Increasing evidence suggested nephrolithiasis has a close linkage with carotid atherosclerosis (CAS), with Randall's plaque (RP) being a precursor to kidney stones. Our study aimed to examine the crosstalk genes and potential molecular mechanisms between RP and CAS. We obtained microarray data for RP and CAS from the Gene Expression Omnibus (GEO) and used weighted gene co-expression network analysis (WGCNA) and differential gene expression (DEG) analysis to identify shared genes. By integrating WGCNA and DEG analysis, Asporin (ASPN) was identified as the key gene connecting RP and CAS, with its diagnostic potential assessed via a receiver operating characteristic (ROC) curve. Immune infiltration studies showed a significant correlation between ASPN and various immune cells in RP and CAS. ASPN was found to be less expressed in RP and CAS tissues compared to normal tissues, as confirmed by immunohistochemistry (IHC) and quantitative reverse-transcription PCR (qRT-PCR). The rat model confirmed the human tissue findings. ASPN can elucidate the shared pathogenic mechanisms underlying the two conditions, including immune response and osteoblast differentiation.

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探索肾结石与颈动脉粥样硬化之间的分子相互作用：作为潜在生物标志物的porin。

越来越多的证据表明，肾结石与颈动脉粥样硬化（CAS）密切相关，而兰德尔斑块（RP）是肾结石的前兆。我们的研究旨在研究 RP 和 CAS 之间的串联基因和潜在的分子机制。我们从基因表达总库（GEO）中获得了RP和CAS的微阵列数据，并使用加权基因共表达网络分析（WGCNA）和差异基因表达分析（DEG）来识别共享基因。通过整合 WGCNA 和 DEG 分析，阿斯匹林（ASPN）被确定为连接 RP 和 CAS 的关键基因，其诊断潜力可通过接收者操作特征曲线（ROC）进行评估。免疫浸润研究显示，ASPN 与 RP 和 CAS 中的各种免疫细胞之间存在显著相关性。免疫组织化学（IHC）和定量反转录 PCR（qRT-PCR）证实，与正常组织相比，ASPN 在 RP 和 CAS 组织中的表达较少。大鼠模型证实了人体组织的研究结果。ASPN 可以阐明这两种疾病的共同致病机制，包括免疫反应和成骨细胞分化。

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来源期刊

Urolithiasis UROLOGY & NEPHROLOGY-

CiteScore

4.50

自引率

6.50%

发文量

期刊介绍： Official Journal of the International Urolithiasis Society The journal aims to publish original articles in the fields of clinical and experimental investigation only within the sphere of urolithiasis and its related areas of research. The journal covers all aspects of urolithiasis research including the diagnosis, epidemiology, pathogenesis, genetics, clinical biochemistry, open and non-invasive surgical intervention, nephrological investigation, chemistry and prophylaxis of the disorder. The Editor welcomes contributions on topics of interest to urologists, nephrologists, radiologists, clinical biochemists, epidemiologists, nutritionists, basic scientists and nurses working in that field. Contributions may be submitted as full-length articles or as rapid communications in the form of Letters to the Editor. Articles should be original and should contain important new findings from carefully conducted studies designed to produce statistically significant data. Please note that we no longer publish articles classified as Case Reports. Editorials and review articles may be published by invitation from the Editorial Board. All submissions are peer-reviewed. Through an electronic system for the submission and review of manuscripts, the Editor and Associate Editors aim to make publication accessible as quickly as possible to a large number of readers throughout the world.