Generation of the TSHSUi002-A induced pluripotent stem cell line from a patient with Peutz-Jeghers syndrome carring STK11 gene mutation

IF 0.8 4区 医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Stem cell research Pub Date : 2024-11-22 DOI:10.1016/j.scr.2024.103618
Weibo Chen , Hongjuan Wang , Kongxi Zhu , Teng Wang , Weihua Yu , Qiong Wu
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Abstract

We report the derivation of an induced pluripotent stem cell (iPSC) line, designated TSHSUi002-A, from a patient with Peutz-Jeghers syndrome carrying a heterozygous c.909C>G mutation in the STK11 gene. The iPSCs were generated through the reprogramming of peripheral blood mononuclear cells using a non-integrating method involving episomal vectors expressing OCT4, SOX2, KLF4, BCL-XL, and c-MYC. These iPSCs exhibit pluripotency markers, are capable of differentiating into cells of all three germ layers in vitro, and maintain a normal karyotype.
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从一名携带 STK11 基因突变的 Peutz-Jeghers 综合征患者身上获得 TSHSUi002-A 诱导多能干细胞系
我们报告了从一名携带STK11基因杂合c.909C>G突变的Peutz-Jeghers综合征患者身上获得的诱导多能干细胞(iPSC)系,命名为TSHSUi002-A。这些 iPSCs 是通过使用表达 OCT4、SOX2、KLF4、BCL-XL 和 c-MYC 的外显子载体的非整合方法对外周血单核细胞进行重编程生成的。这些 iPSCs 具有多能性标记,能够在体外分化成所有三个生殖层的细胞,并保持正常的核型。
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来源期刊
Stem cell research
Stem cell research 生物-生物工程与应用微生物
CiteScore
2.20
自引率
8.30%
发文量
338
审稿时长
55 days
期刊介绍: Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.
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