Potential Biomarkers for Neurobrucellosis Diagnosis and Treatment Based on Cerebrospinal Fluid Metabolomics Analysis

IF 1.8 3区化学 Q4 BIOCHEMICAL RESEARCH METHODS Rapid Communications in Mass Spectrometry Pub Date : 2024-11-27 DOI:10.1002/rcm.9946

Meiling Liu, Shujun Shi, Yuzhou Guo, Menghan Lv, Xuan Feng, Ting Liu, Hao Yang, Zhelin Zhang

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Abstract

Background

Zhenzhu Tongluo pills (ZZTL) have been utilized for the treatment of neurobrucellosis (NB) in the Inner Mongolia region of China. However, the specific mechanism underlying the neuroprotective effects of ZZTL in NB remains insufficiently explored. Therefore, this study aimed to analyze the metabolite profiles across different groups to identify potential biomarkers for the diagnosis and treatment of NB.

Methods

LC–MS analysis was used to screen differential metabolites in cerebrospinal fluid (CSF) samples from control subjects, NB patients, and NB patients undergoing ZZTL therapy.

Results

We identified 225 common metabolites across two comparison groups (NB vs. control group and NB + ZZTL vs. NB group). Among these metabolites, 138 were downregulated, and 87 were upregulated in the NB group compared to the control group, while the levels of these metabolites were reversed by ZZTL therapy. Creatinine exhibited the highest VIP score in both comparison groups, with elevated levels observed in CSF samples from NB patients compared to controls; however, its levels were reduced following ZZTL therapy. Additionally, hypoxanthine and uric acids levels were also increased in CSF samples from NB patients, indicating dysregulation of purine metabolism in NB; however, these changes were reversed by ZZTL. Furthermore, pantothenic acid (vitamin B5) and niacinamide (vitamin B3) levels were decreased in CSF samples from NB patients, suggesting a potential link between NB and vitamin B3 and B5 deficiency; however, these changes were reversed by ZZTL.

Conclusion

Collectively, CSF metabolomics may effectively differentiate NB patients from control subjects, providing valuable insights for NB diagnosis and treatment.

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基于脑脊液代谢组学分析的神经布鲁氏菌病诊断和治疗的潜在生物标记物

背景：在中国内蒙古地区，镇痫通络丸（ZZTL）一直被用于治疗神经布鲁氏菌病（NB）。然而，ZZTL对NB神经保护作用的具体机制仍未得到充分探讨。因此，本研究旨在分析不同群体的代谢物谱，以确定诊断和治疗NB的潜在生物标志物：方法：采用LC-MS分析筛选对照组、NB患者和接受ZZTL治疗的NB患者脑脊液（CSF）样本中的不同代谢物：结果：我们在两个对比组（NB 组与对照组、NB + ZZTL 组与 NB 组）中发现了 225 种常见代谢物。在这些代谢物中，与对照组相比，NB组有138种代谢物下调，87种代谢物上调，而ZZTL治疗可逆转这些代谢物的水平。在两个对比组中，肌酐的VIP得分最高，在NB患者的脑脊液样本中观察到肌酐水平高于对照组；然而，在ZZTL治疗后，肌酐水平有所降低。此外，NB 患者脑脊液样本中的次黄嘌呤和尿酸水平也有所升高，这表明 NB 中的嘌呤代谢失调；然而，ZZTL 可逆转这些变化。此外，NB患者脑脊液样本中泛酸（维生素B5）和烟酰胺（维生素B3）水平降低，表明NB与维生素B3和B5缺乏之间存在潜在联系；然而，ZZTL可逆转这些变化：总之，脑脊液代谢组学可有效区分NB患者和对照组，为NB的诊断和治疗提供有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rapid Communications in Mass Spectrometry 化学-分析化学

CiteScore

4.10

自引率

5.00%

发文量

219

审稿时长

2.6 months

期刊介绍： Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.