First Report of Carbapenem-Resistant Klebsiella michiganensis Co-Harboring blaKPC-2 and TmexCD2-ToprJ2 Isolated from Wastewater at a Tertiary Hospital in Beijing.
{"title":"First Report of Carbapenem-Resistant <i>Klebsiella michiganensis</i> Co-Harboring <i>bla</i> <sub>KPC-2</sub> and <i>TmexCD2-ToprJ2</i> Isolated from Wastewater at a Tertiary Hospital in Beijing.","authors":"Jiamin Long, Jiali Chen, Yue Yuan, Shaozhen Yang, Xinya Xie, Xuelian Wu, Yuan Liu, Jinpeng Guo, Yong Chen, Changjun Wang, Xiong Liu","doi":"10.2147/IDR.S448256","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Klebsiella michiganensis</i> is an emerging human pathogen that causes nosocomial infections. Its prevalence and spread in the environment should not be ignored. This study identified and characterized <i>Klebsiella michiganensis</i> co-harboring <i>bla</i> <sub>KPC-2</sub> and <i>TmexCD2-ToprJ2</i> in hospital wastewater samples.</p><p><strong>Methods: </strong>Twelve <i>K. michiganensis</i> strains were isolated from wastewater samples collected at a tertiary hospital in Beijing, China. The genomic characteristics of <i>K. michiganensis</i> strains were analyzed using whole-genome sequences, providing information on the comparison between the genome of <i>K. michiganensis</i> strains and the reference genome, antibiotic resistance genes (ARGs), virulence genes, secretion systems, and mobile genetic elements (plasmids, insertion sequences [ISs], and prophages).</p><p><strong>Results: </strong>Genome analysis showed that the twelve multi-drug resistant (MDR) strains carried a variety of ARGs and virulence genes, as well as four macromolecular secretion systems (T1SS, T2SS, T5aSS, T5bSS, and T4aP). The genetic environments of both the <i>TmexCD2-ToprJ2</i> gene cluster and <i>bla</i> <sub>KPC-2</sub> gene contained ISs. The plasmids carrying <i>TmexCD2-ToprJ2</i> gene cluster of nine strains in clade 1 and two strains in clade 2 were annotated as IncR plasmid and rep_cluster_1254 type, respectively. The plasmids carrying <i>bla</i> <sub>KPC-2</sub> in 10 strains in clade 1 were identified as IncU, and the plasmids carrying <i>bla</i> <sub>KPC-2</sub> in the k11 and k12 strains in clade 2 were IncU and IncX6. The phylogenetic tree and heatmap revealed that the secretion system of type VI (T6SSi) existed in 10 strains in clade 1, and Type IV (T4SS) only existed in the k11 strain in clade 2. In addition, <i>K. michiganensis</i> strains carried 13 plasmids, 14 ISs, and 138 prophages.</p><p><strong>Conclusion: </strong>In this study, the whole genome sequencing demonstrated the diversity of <i>K. michiganensis</i> genome despite 12 <i>K. michiganensis</i> strains from a hospital wastewater, which lays the foundation for further genetic research and drug resistance gene transmission.</p>","PeriodicalId":13577,"journal":{"name":"Infection and Drug Resistance","volume":"17 ","pages":"5117-5128"},"PeriodicalIF":2.9000,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589776/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infection and Drug Resistance","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/IDR.S448256","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Klebsiella michiganensis is an emerging human pathogen that causes nosocomial infections. Its prevalence and spread in the environment should not be ignored. This study identified and characterized Klebsiella michiganensis co-harboring blaKPC-2 and TmexCD2-ToprJ2 in hospital wastewater samples.
Methods: Twelve K. michiganensis strains were isolated from wastewater samples collected at a tertiary hospital in Beijing, China. The genomic characteristics of K. michiganensis strains were analyzed using whole-genome sequences, providing information on the comparison between the genome of K. michiganensis strains and the reference genome, antibiotic resistance genes (ARGs), virulence genes, secretion systems, and mobile genetic elements (plasmids, insertion sequences [ISs], and prophages).
Results: Genome analysis showed that the twelve multi-drug resistant (MDR) strains carried a variety of ARGs and virulence genes, as well as four macromolecular secretion systems (T1SS, T2SS, T5aSS, T5bSS, and T4aP). The genetic environments of both the TmexCD2-ToprJ2 gene cluster and blaKPC-2 gene contained ISs. The plasmids carrying TmexCD2-ToprJ2 gene cluster of nine strains in clade 1 and two strains in clade 2 were annotated as IncR plasmid and rep_cluster_1254 type, respectively. The plasmids carrying blaKPC-2 in 10 strains in clade 1 were identified as IncU, and the plasmids carrying blaKPC-2 in the k11 and k12 strains in clade 2 were IncU and IncX6. The phylogenetic tree and heatmap revealed that the secretion system of type VI (T6SSi) existed in 10 strains in clade 1, and Type IV (T4SS) only existed in the k11 strain in clade 2. In addition, K. michiganensis strains carried 13 plasmids, 14 ISs, and 138 prophages.
Conclusion: In this study, the whole genome sequencing demonstrated the diversity of K. michiganensis genome despite 12 K. michiganensis strains from a hospital wastewater, which lays the foundation for further genetic research and drug resistance gene transmission.
期刊介绍：
About Journal
Editors
Peer Reviewers
Articles
Article Publishing Charges
Aims and Scope
Call For Papers
ISSN: 1178-6973
Editor-in-Chief: Professor Suresh Antony
An international, peer-reviewed, open access journal that focuses on the optimal treatment of infection (bacterial, fungal and viral) and the development and institution of preventative strategies to minimize the development and spread of resistance.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
