Genetic and Phenotypic Characterization of Botrytis Populations from Economic and Wild Host Plants in Iran.

IF 4.2 2区生物学 Q2 MICROBIOLOGY Journal of Fungi Pub Date : 2024-11-02 DOI:10.3390/jof10110764

Sepideh Fekrikohan, Bahram Sharifnabi, Mohammad Javan-Nikkhah, Stefania Pollastro, Francesco Faretra, Rita Milvia De Miccolis Angelini

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Abstract

Grey mould disease, caused by various Botrytis species, poses a significant threat to important plants worldwide. This study aimed to characterize Botrytis populations on strawberry and roses, economically relevant host plants, and raspberry, used as a representative of wild plants, in Iran. A total of 389 isolates were collected and analyzed based on morphological features and haplotyping using molecular markers, transposable elements (Boty and Flipper), and fungicide response. Moreover, 60 isolates were used for phylogenetic analysis based on the rpb2 gene, and 16 selected isolates from each clade were further characterized using the g3pdh, hsp60, and nep2 genes. The results revealed the presence of three distinct species, Botrytis cinerea, Botrytis sinoviticola, and Botrytis prunorum, among the sampled isolates. Additionally, this study reports for the first time the presence of B. sinoviticola on strawberry and isolates belonging to B. cinerea group S in Iran. These findings provide insights into the diversity and composition of Botrytis populations on Iranian host plants.

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伊朗经济寄主植物和野生寄主植物灰霉病菌种群的遗传和表型特征。

由各种灰霉病菌引起的灰霉病对全球重要植物构成了严重威胁。本研究旨在分析伊朗草莓和玫瑰（经济上相关的寄主植物）以及覆盆子（野生植物的代表）上灰霉菌种群的特征。共收集了 389 个分离株，并根据形态特征和使用分子标记的单倍型分析、转座元件（Boty 和 Flipper）以及杀菌剂反应进行了分析。此外，还根据 rpb2 基因对 60 个分离株进行了系统进化分析，并使用 g3pdh、hsp60 和 nep2 基因对每个支系中选出的 16 个分离株进行了进一步鉴定。研究结果表明，在采样的分离株中存在三个不同的种，分别是银色葡萄孢菌（Botrytis cinerea）、山葡萄孢菌（Botrytis sinoviticola）和春葡萄孢菌（Botrytis prunorum）。此外，本研究首次报告了伊朗草莓上存在的 B. sinoviticola 和属于 S 组的 B. cinerea 分离物。这些发现有助于深入了解伊朗寄主植物上灰霉病菌种群的多样性和组成。

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来源期刊

Journal of Fungi Medicine-Microbiology (medical)

CiteScore

6.70

自引率

14.90%

发文量

1151

审稿时长

11 weeks

期刊介绍： Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.