Optical Clearing of Ex Vivo Adipose Tissue

IF 2.2 3区医学 Q2 DERMATOLOGY Lasers in Surgery and Medicine Pub Date : 2024-11-26 DOI:10.1002/lsm.23860

Irina Yu. Yanina, Elina A. Genina, Daria K. Tuchina, Polina A. Timoshina, Nikita A. Navolokin, Alla B. Bucharskaya, Galina N. Maslyakova, Valery V. Tuchin

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Abstract

Objectives

The paper focuses on the development of technology of adipose tissue optical clearing using different complex hyperosmotic optical clearing agents and tissue permeability enhancers.

Methods

To quantify optical clearing efficiency, reduced scattering coefficient was estimated from the ex vivo spatially resolved backreflectance measurements using a multi-distant fiber optical device. Tissue morphology modification was monitored with the help of histological studies.

Results

Kinetics and efficiency of the optical clearing were evaluated for ex vivo abdominal fat tissue at action of dimethyl sulfoxide, diatrizoic acid, metrizoic acid, sucrose, and fructose solutions accompanied by tissue permeability enhancers, such as various modes of fractional laser microablation and sonophoresis and their combinations. Histological studies allowed us to find agents that cause minimal morphological changes of the adipose tissue.

Conclusions

Maximal optical clearing efficiency of 83.5% was observed for the samples subjected to action of the fructose-ethanol solution during 90 min in combination with gentle modes of fractional laser microablation and sonophoresis.

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体内脂肪组织的光学清除。

目的本文重点研究使用不同的复合高渗光学清除剂和组织渗透性增强剂对脂肪组织进行光学清除的技术开发：方法：为了量化光学清除效率，使用多远光纤光学设备通过活体外空间分辨背反射测量来估算降低的散射系数。在组织学研究的帮助下，对组织形态的改变进行了监测：结果：在二甲基亚砜、二苯甲酸、偏苯甲酸、蔗糖和果糖溶液以及组织渗透性增强剂（如各种模式的点阵激光微消融和声波电泳及其组合）的作用下，对体内腹部脂肪组织的光学清除动力学和效率进行了评估。通过组织学研究，我们找到了能使脂肪组织形态变化最小的药剂：结论：果糖乙醇溶液与温和的点阵激光微消融和声波电泳结合使用 90 分钟后，观察到样本的最大光学清除率为 83.5%。

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来源期刊

Lasers in Surgery and Medicine 医学-外科

CiteScore

5.40

自引率

12.50%

发文量

119

审稿时长

1 months

期刊介绍： Lasers in Surgery and Medicine publishes the highest quality research and clinical manuscripts in areas relating to the use of lasers in medicine and biology. The journal publishes basic and clinical studies on the therapeutic and diagnostic use of lasers in all the surgical and medical specialties. Contributions regarding clinical trials, new therapeutic techniques or instrumentation, laser biophysics and bioengineering, photobiology and photochemistry, outcomes research, cost-effectiveness, and other aspects of biomedicine are welcome. Using a process of rigorous yet rapid review of submitted manuscripts, findings of high scientific and medical interest are published with a minimum delay.