Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against Fusobacterium nucleatum Subspecies.

IF 3.3 3区医学 Q2 MICROBIOLOGY Pathogens Pub Date : 2024-11-19 DOI:10.3390/pathogens13111016

Jianglan Li, Takayuki Nambu, Chao Wang, Hiroki Takigawa, Hugo Maruyama, Chiho Mashimo, Toshinori Okinaga

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Abstract

Fusobacterium nucleatum (F. nucleatum), a key pathogen implicated in periodontal disease, contributes to oral biofilm maturation and is linked to development of systemic diseases like colorectal cancer and liver cirrhosis. Photodynamic therapy (PDT) combined with 5-aminolevulinic acid (5-ALA) treatment (ALA-PDT) selectively targets F. nucleatum by inducing porphyrin accumulation. The bactericidal effect of red light-based PDT on F. nucleatum has not been evaluated previously. This study investigates the effect of ALA-PDT using red light-emitting diode (LED) light on F. nucleatum subspecies and their porphyrin accumulation. F. nucleatum subspecies were cultured with varying concentrations of 5-ALA under anaerobic conditions. Porphyrin accumulation was measured via fluorescence spectroscopy, and colony-forming units were measured to determine bacterial viability post-treatment. Additionally, other subspecies responded well to 0.01% 5-ALA, and uroporphyrin I accumulation correlated with bacterial death, revealing optimal bactericidal conditions. These results suggest that optimizing light intensity and 5-ALA concentration can significantly enhance the therapeutic potential of ALA-PDT in oral healthcare.

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优化基于红光的光动力疗法，对核分枝杆菌亚种产生有效杀菌作用

核团镰刀菌（F. nucleatum）是牙周病的主要病原体，它有助于口腔生物膜的成熟，并与结肠直肠癌和肝硬化等全身性疾病的发展有关。光动力疗法（PDT）结合 5-氨基乙酰丙酸（5-ALA）治疗（ALA-PDT）通过诱导卟啉积累，选择性地针对核酸酵母菌。基于红光的 PDT 对 F. nucleatum 的杀菌效果此前尚未进行过评估。本研究探讨了使用红色发光二极管（LED）光的 ALA-PDT 对 F. nucleatum 亚种及其卟啉积累的影响。在厌氧条件下，用不同浓度的 5-ALA 培养 F. nucleatum 亚种。通过荧光光谱测定卟啉的积累，并测定菌落形成单位，以确定处理后细菌的存活率。此外，其他亚种对 0.01% 5-ALA 反应良好，尿卟啉 I 的积累与细菌死亡相关，揭示了最佳杀菌条件。这些结果表明，优化光照强度和 5-ALA 浓度可显著提高 ALA-PDT 在口腔保健中的治疗潜力。

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来源期刊

Pathogens Medicine-Immunology and Allergy

CiteScore

6.40

自引率

8.10%

发文量

1285

审稿时长

17.75 days

期刊介绍： Pathogens (ISSN 2076-0817) publishes reviews, regular research papers and short notes on all aspects of pathogens and pathogen-host interactions. There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental and/or methodical details must be provided for research articles.