Comparative lipidome and transcriptome provide novel insights into zero-valent iron nanoparticle-treated Fremyella diplosiphon.

IF 3.8 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Scientific Reports Pub Date : 2024-11-26 DOI:10.1038/s41598-024-79780-7

Yavuz S Yalcin, Samson Gichuki, Huan Chen, Anithachristy Sigamani Arumanayagam, Shyama Malika Malwalage, Viji Sitther

{"title":"Comparative lipidome and transcriptome provide novel insights into zero-valent iron nanoparticle-treated Fremyella diplosiphon.","authors":"Yavuz S Yalcin, Samson Gichuki, Huan Chen, Anithachristy Sigamani Arumanayagam, Shyama Malika Malwalage, Viji Sitther","doi":"10.1038/s41598-024-79780-7","DOIUrl":null,"url":null,"abstract":"Understanding the intricate interplay between nanoparticle-mediated cyanobacterial interactions is pivotal in elucidating their impact on the transcriptome and lipidome. In the present study, total fatty acid methyl esters (FAMEs) in the wild-type (B481-WT) and transformant (B481-SD) Fremyella diplosiphon strains treated with nanoscale zero-valent iron nanoparticles (nZVIs) were characterized, and transcriptome changes analyzed. Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry revealed a 20-25% higher percentage of FAMEs in nZVI-treated F. diplosiphon strain B481-SD compared to B481-WT. Accumulation of alkanes was significantly higher (> 1.4 times) in both strains treated with 25.6 mg L-1 nZVIs compared to the untreated control. In addition, we observed significantly higher levels of monounsaturated FAMEs (11%) in B481-WT in 3.2 (11.34%) and 25.6 mg L-1 (11.22%) nZVI-treated cells when compared to the untreated control (7%). Analysis of the F. diplosiphon transcriptome treated with 3.2 mg L-1 revealed a total of 1811 and 1651 genes that were differentially expressed in B481-SD and B481-WT respectively. While the expression of iron uptake and ion channel genes was downregulated, genes coding for photosynthesis, pigment, and antioxidant enzymes were significantly (p < 0.05) upregulated in B481-SD treated with 3.2 mg L-1 nZVIs compared to the untreated control. This study on essential FAMEs and regulation of genes in nZVI-treated F. diplosiphon strains provides a molecular framework for optimization of metabolic pathways in this model species.","PeriodicalId":21811,"journal":{"name":"Scientific Reports","volume":"14 1","pages":"29380"},"PeriodicalIF":3.8000,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientific Reports","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.1038/s41598-024-79780-7","RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Understanding the intricate interplay between nanoparticle-mediated cyanobacterial interactions is pivotal in elucidating their impact on the transcriptome and lipidome. In the present study, total fatty acid methyl esters (FAMEs) in the wild-type (B481-WT) and transformant (B481-SD) Fremyella diplosiphon strains treated with nanoscale zero-valent iron nanoparticles (nZVIs) were characterized, and transcriptome changes analyzed. Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry revealed a 20-25% higher percentage of FAMEs in nZVI-treated F. diplosiphon strain B481-SD compared to B481-WT. Accumulation of alkanes was significantly higher (> 1.4 times) in both strains treated with 25.6 mg L^-1 nZVIs compared to the untreated control. In addition, we observed significantly higher levels of monounsaturated FAMEs (11%) in B481-WT in 3.2 (11.34%) and 25.6 mg L^-1 (11.22%) nZVI-treated cells when compared to the untreated control (7%). Analysis of the F. diplosiphon transcriptome treated with 3.2 mg L^-1 revealed a total of 1811 and 1651 genes that were differentially expressed in B481-SD and B481-WT respectively. While the expression of iron uptake and ion channel genes was downregulated, genes coding for photosynthesis, pigment, and antioxidant enzymes were significantly (p < 0.05) upregulated in B481-SD treated with 3.2 mg L^-1 nZVIs compared to the untreated control. This study on essential FAMEs and regulation of genes in nZVI-treated F. diplosiphon strains provides a molecular framework for optimization of metabolic pathways in this model species.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

脂质体和转录组的比较为了解经零价铁纳米粒子处理的双子叶弗氏菌提供了新的视角。

了解纳米粒子介导的蓝藻相互作用之间错综复杂的相互作用对于阐明其对转录组和脂质组的影响至关重要。本研究表征了经纳米级零价铁纳米颗粒（nZVIs）处理的野生型（B481-WT）和转化型（B481-SD）双子叶弗氏蓝藻菌株的总脂肪酸甲酯（FAMEs），并分析了转录组的变化。综合二维气相色谱-飞行时间质谱分析表明，与 B481-WT 相比，经 nZVI 处理的虹彩褐飞虱菌株 B481-SD 的 FAMEs 百分比高出 20-25%。与未处理的对照组相比，经 25.6 mg L-1 nZVIs 处理的两个菌株中烷烃的积累量都显著较高（> 1.4 倍）。此外，与未经处理的对照组（7%）相比，我们观察到经 3.2（11.34%）和 25.6 mg L-1 nZVI 处理的 B481-WT 细胞中单不饱和脂肪酸含量（11%）明显更高。对经 3.2 mg L-1 处理的双子叶蝇转录组的分析表明，在 B481-SD 和 B481-WT 中分别有 1811 和 1651 个基因表达不同。与未处理的对照组相比，铁吸收基因和离子通道基因的表达下调，而光合作用、色素和抗氧化酶编码基因的表达则显著（p -1 nZVIs）。这项关于经 nZVI 处理的双子叶蝇蛆菌株中必需的 FAMEs 和基因调控的研究为优化该模式物种的代谢途径提供了一个分子框架。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

期刊介绍： We publish original research from all areas of the natural sciences, psychology, medicine and engineering. You can learn more about what we publish by browsing our specific scientific subject areas below or explore Scientific Reports by browsing all articles and collections. Scientific Reports has a 2-year impact factor: 4.380 (2021), and is the 6th most-cited journal in the world, with more than 540,000 citations in 2020 (Clarivate Analytics, 2021). •Engineering Engineering covers all aspects of engineering, technology, and applied science. It plays a crucial role in the development of technologies to address some of the world''s biggest challenges, helping to save lives and improve the way we live. •Physical sciences Physical sciences are those academic disciplines that aim to uncover the underlying laws of nature — often written in the language of mathematics. It is a collective term for areas of study including astronomy, chemistry, materials science and physics. •Earth and environmental sciences Earth and environmental sciences cover all aspects of Earth and planetary science and broadly encompass solid Earth processes, surface and atmospheric dynamics, Earth system history, climate and climate change, marine and freshwater systems, and ecology. It also considers the interactions between humans and these systems. •Biological sciences Biological sciences encompass all the divisions of natural sciences examining various aspects of vital processes. The concept includes anatomy, physiology, cell biology, biochemistry and biophysics, and covers all organisms from microorganisms, animals to plants. •Health sciences The health sciences study health, disease and healthcare. This field of study aims to develop knowledge, interventions and technology for use in healthcare to improve the treatment of patients.