QTL-seq and QTL mapping identify a new locus for Cercospora leaf spot (Cercospora canescens) resistance in mungbean (Vigna radiata) and a cluster of Receptor-like protein 12 (RLP12) genes as candidate genes for the resistance.

IF 4.4 1区 农林科学 Q1 AGRONOMY Theoretical and Applied Genetics Pub Date : 2024-11-27 DOI:10.1007/s00122-024-04782-y
Makawan Srichan, Kularb Laosatit, Yun Lin, Xingxing Yuan, Xin Chen, Prakit Somta
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Abstract

Key message: QTL-seq, linkage mapping, and whole-genome resequencing revealed a new locus (qCLS5.1) controlling Cercospora canescens resistance in mungbean and Receptor-like protein 12 (RLP12) genes as candidate genes for the resistance. Cercospora leaf spot (CLS) disease, caused by Cercospora canescens, is a common disease of mungbean (Vigna radiata). In this study, the genetics of CLS resistance was investigated in a new source of resistance (accession V2817) and the resistance was finely mapped to identify candidate genes. F2 and F2:3 populations of the cross V1197 (susceptible) × V2718 and a BC1F1 population of the cross V1197 × (V1197 × V2817) were used in this study. Segregation analysis suggested that the resistance is controlled by a single dominant gene. QTL-seq using F2 individuals revealed that a single QTL (designated qCLS5.1) on chromosome 5 controlled the resistance. The qCLS5.1 was confirmed in the F2:3 and BC1F1 populations by QTL analysis. Fine mapping using 978 F2 individuals localized qCLS5.1 to a 48.94 Kb region containing three tandemly duplicated Receptor-like protein 12 (RLP12) genes. Whole-genome resequencing and alignment of V1197 and V2817 revealed polymorphisms causing amino acid changes and premature stop codons in the three RLP12 genes. Collectively, these results show that qCLS5.1 is a new locus for CLS resistance in mungbean, and a cluster of RLP12 genes are candidate genes for the resistance. The new locus qCLS5.1 will be useful for molecular breeding of durable CLS-resistant mungbean cultivars.

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QTL-seq和QTL图谱确定了绿豆(Vigna radiata)抗Cercospora叶斑病(Cercospora canescens)的新位点,以及作为抗性候选基因的受体样蛋白12(RLP12)基因簇。
关键信息:QTL-seq、连锁图谱和全基因组重测序揭示了一个控制绿豆Cercospora canescens抗性的新位点(qCLS5.1),以及作为抗性候选基因的受体样蛋白12(RLP12)基因。由Cercospora canescens引起的Cercospora叶斑病(CLS)是绿豆(Vigna radiata)的一种常见病害。本研究对一个新的抗性来源(登录号 V2817)进行了 CLS 抗性遗传学调查,并对抗性进行了精细图谱绘制,以确定候选基因。本研究使用了 V1197(易感)×V2718 杂交的 F2 和 F2:3 群体以及 V1197 ×(V1197 × V2817)杂交的 BC1F1 群体。分离分析表明,抗性由单一显性基因控制。利用F2个体进行的QTL-seq分析表明,5号染色体上的单个QTL(命名为qCLS5.1)控制着抗性。通过 QTL 分析,在 F2:3 和 BC1F1 群体中确认了 qCLS5.1。利用 978 个 F2 群体进行的精细作图将 qCLS5.1 定位在一个 48.94 Kb 的区域,该区域包含三个串联重复的类受体蛋白 12(RLP12)基因。V1197 和 V2817 的全基因组重测序和比对显示,多态性导致三个 RLP12 基因中的氨基酸变化和过早终止密码子。这些结果表明,qCLS5.1是绿豆抗CLS的新位点,RLP12基因簇是抗性的候选基因。新基因座 qCLS5.1 将有助于耐久抗 CLS 绿豆品种的分子育种。
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来源期刊
CiteScore
9.60
自引率
7.40%
发文量
241
审稿时长
2.3 months
期刊介绍: Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
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