Immune Response Modulation by HPV16 Oncoproteins in Lung Cancer: Insights from Clinical and In Vitro Investigations.

IF 3.5 3区医学 Q2 VIROLOGY Viruses-Basel Pub Date : 2024-11-04 DOI:10.3390/v16111731

Bianca de França São Marcos, Daffany Luana Dos Santos, Georon Ferreira de Sousa, Leonardo Carvalho de Oliveira Cruz, Bárbara Rafaela da Silva Barros, Matheus Gardini Amâncio Marques de Sena, Vanessa Emanuelle Pereira Santos, Talita Helena de Araújo Oliveira, Cristiane Moutinho Lagos de Melo, Antonio Carlos de Freitas

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Abstract

Lung cancer has the highest mortality rates worldwide, and Human Papillomavirus (HPV) has been associated with its carcinogenesis. In this study, HPV16 genes' expressions were investigated in patient samples, along with the immunological response promoted by lymphocytes and monocytes in A549 cells transfected with HPV oncogenes and co-cultured with PBMC. An increase in the expression of E5 was observed in the patients' samples. In the in vitro analysis, a decrease in the number of monocytes and cytotoxic cells was observed when co-stimulated by E6 and E7, and it promoted an increase in the Th2 profile. In contrast, the high proliferation of cytotoxic cells in A549 cells transfected with E5, associated with the high expression of costimulatory molecules in monocytes, suggests a low capacity of E5 to inhibit the presentation of antigens by antigen-presenting cells (APC) and a possible use of E5 in future therapeutic strategies against lung cancers associated with HPV.

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肺癌中 HPV16 肿瘤蛋白的免疫反应调节：临床和体外研究的启示。

肺癌是全球死亡率最高的癌症，而人类乳头瘤病毒（HPV）与肺癌的发生有关。本研究调查了患者样本中 HPV16 基因的表达情况，以及淋巴细胞和单核细胞在转染 HPV 致癌基因并与 PBMC 共同培养的 A549 细胞中促进的免疫反应。在患者样本中观察到 E5 的表达增加。在体外分析中，观察到在 E6 和 E7 的共同刺激下，单核细胞和细胞毒性细胞的数量减少，并促进 Th2 特征的增加。相比之下，转染了 E5 的 A549 细胞中细胞毒性细胞的高增殖与单核细胞中 costimulatory 分子的高表达有关，这表明 E5 抑制抗原递呈细胞（APC）递呈抗原的能力较低，E5 有可能被用于未来针对与 HPV 相关的肺癌的治疗策略中。

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来源期刊

Viruses-Basel VIROLOGY-

CiteScore

7.30

自引率

12.80%

发文量

2445

审稿时长

1 months

期刊介绍： Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.