Biological Characterization and Evaluation of the Therapeutic Value of Vibrio Phages 4141 and MJW Isolated from Clinical and Sewage Water Samples of Kolkata.

IF 3.8 3区医学 Q2 VIROLOGY Viruses-Basel Pub Date : 2024-11-06 DOI:10.3390/v16111741

Sanjoy Biswas, Devendra Nath Tewari, Alok Kumar Chakrabarti, Shanta Dutta

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Abstract

The growing prevalence of antimicrobial resistance (AMR) necessitates the development of new treatment methods to combat diseases like cholera. Lytic bacteriophages are viruses that specifically target and lyse bacteria upon infection, making them a possible treatment option for multi-drug-resistant pathogens. The current study investigated the potential role of bacteriophages isolated from clinical stool and sewage water samples in treating multi-drug-resistant Vibrio cholerae infection, finding that over 95% of the strains were susceptible. Whole-genome sequencing (WGS) analysis revealed that both Vibrio phage 4141 (4141) and Vibrio phage MJW (MJW) contain double-stranded DNA genomes consisting of 38,498 bp (43% GC) and 49,880 bp (42.5% GC) with 46 and 64 open reading frames (ORFs), respectively. Transmission electron microscope (TEM) and WGS analysis of Vibrio phage 4141 and Vibrio phage MJW validated that they are classified under the family Autographiviridae and Zobellviridae, respectively. Furthermore, both the phages showed highly significant biofilm degradation properties. The characterization of the phages and their strict host range, high spectrum of lytic ability, high efficiency of biofilm degradation, and close genetic similarity to the therapeutic phages indicates that these phages may be useful for therapeutic purposes for treating MDR V. cholerae infection in the future.

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从加尔各答临床和污水样本中分离出的弧菌噬菌体 4141 和 MJW 的生物学特征和治疗价值评估。

抗菌药耐药性（AMR）日益普遍，因此有必要开发新的治疗方法来防治霍乱等疾病。溶解性噬菌体是一种病毒，能在感染后特异性地靶向细菌并使其溶解，从而使其成为治疗多重耐药性病原体的一种可能选择。目前的研究调查了从临床粪便和污水样本中分离出的噬菌体在治疗多重耐药霍乱弧菌感染中的潜在作用，发现超过 95% 的菌株对噬菌体敏感。全基因组测序（WGS）分析表明，弧菌噬菌体 4141（4141）和弧菌噬菌体 MJW（MJW）都含有双链 DNA 基因组，分别由 38,498 bp（43% GC）和 49,880 bp（42.5% GC）组成，具有 46 个和 64 个开放阅读框（ORF）。对弧菌噬菌体 4141 和弧菌噬菌体 MJW 的透射电子显微镜（TEM）和 WGS 分析验证了它们分别归属于 Autographiviridae 和 Zobellviridae 科。此外，这两种噬菌体都具有非常显著的生物膜降解特性。这些噬菌体的特征及其严格的宿主范围、高频谱的溶解能力、高效的生物膜降解能力以及与治疗用噬菌体的基因相似性表明，这些噬菌体将来可能会用于治疗 MDR 型霍乱弧菌感染。

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来源期刊

Viruses-Basel VIROLOGY-

CiteScore

7.30

自引率

12.80%

发文量

2445

审稿时长

1 months

期刊介绍： Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.