Serum microRNA 143 and 223 Gene Expression Profiles as Potential Biomarkers in Individuals with Hepatitis and COVID-19.

IF 3.8 3区 医学 Q2 VIROLOGY Viruses-Basel Pub Date : 2024-11-04 DOI:10.3390/v16111734
Lucas Lima da Silva, Luciane Almeida Amado Leon, Otacílio da Cruz Moreira, Wagner Luis da Costa Nunes Pimentel Coelho, Vanessa Duarte da Costa, Claudia Alexandra Pontes Ivantes, Priscila Pollo-Flores, Lia Laura Lewis-Ximenez, Vanessa Salete de Paula, Livia Melo Villar
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Abstract

MicroRNAs (miRNAs) can act as biomarkers and descriptors of the association between infections and other diseases, such as hepatitis and COVID-19. This study aims to investigate the role of miRNA serum expression according to laboratory data concerning hepatitis and COVID-19. Seventy individuals recruited in Southern and Southeastern Brazil donated serum samples and were divided into four groups: (i) 20 negative subjects, (ii) 20 presenting hepatitis, (iii) 19 with COVID-19 and (iv) 11 with hepatitis and COVID-19. Three miRNAs (miR-122, miR-143 and miR-223) were evaluated using real-time PCR. Hematological and biochemical markers were also analyzed. MiR-143 and miR-223 were downregulated among the hepatitis/COVID-19 group (p < 0.05). A positive correlation was observed between miR-223 and lymphocytes. There was a negative correlation between alanine transaminase (ALT) and aspartate transaminase (AST) for miR-143 and miR-223 and gamma-glutamyl transferase (GGT), alkaline phosphatase (AP) and neutrophil/lymphocyte ratio (NLR) only for miR-223 (p < 0.05). For hepatic fibrosis (FIB-4), miR-122 and miR-143 had a greater association and miR-223 was more associated with a history of vaccination against COVID-19. MicroRNAs 143 and 223 could be useful as biomarkers for hepatitis coinfection with COVID-19.

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血清 microRNA 143 和 223 基因表达谱作为肝炎和 COVID-19 患者的潜在生物标记物。
微小RNA(miRNA)可作为生物标记物和感染与其他疾病(如肝炎和COVID-19)之间关联的描述因子。本研究旨在根据有关肝炎和 COVID-19 的实验室数据,研究 miRNA 血清表达的作用。在巴西南部和东南部招募的 70 人捐献了血清样本,并被分为四组:(i) 20 名阴性受试者,(ii) 20 名患有肝炎的受试者,(iii) 19 名患有 COVID-19 的受试者,(iv) 11 名患有肝炎和 COVID-19 的受试者。使用实时 PCR 评估了三种 miRNA(miR-122、miR-143 和 miR-223)。同时还分析了血液学和生化指标。在肝炎/COVID-19 组中,miR-143 和 miR-223 下调(p < 0.05)。miR-223 与淋巴细胞呈正相关。miR-143 和 miR-223 与丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)呈负相关,而 miR-223 仅与γ-谷氨酰转移酶(GGT)、碱性磷酸酶(AP)和中性粒细胞/淋巴细胞比率(NLR)呈负相关(p < 0.05)。对于肝纤维化(FIB-4),miR-122 和 miR-143 的相关性更大,而 miR-223 与 COVID-19 疫苗接种史的相关性更大。微RNA 143和223可作为COVID-19肝炎合并感染的生物标志物。
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来源期刊
Viruses-Basel
Viruses-Basel VIROLOGY-
CiteScore
7.30
自引率
12.80%
发文量
2445
审稿时长
1 months
期刊介绍: Viruses (ISSN 1999-4915) is an open access journal which provides an advanced forum for studies of viruses. It publishes reviews, regular research papers, communications, conference reports and short notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. We also encourage the publication of timely reviews and commentaries on topics of interest to the virology community and feature highlights from the virology literature in the ''News and Views'' section. Electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.
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