Amygdalin inhibits endometrial stromal cell proliferation, migration, and invasion in endometriosis mice via inhibiting Wnt/β-catenin signaling

IF 2.9 4区生物学 Q3 CELL BIOLOGY Journal of Molecular Histology Pub Date : 2024-11-29 DOI:10.1007/s10735-024-10301-6

Miaomiao Yu, Lu Yang, Yonghong Pei, Mei Xu

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Abstract

To explore the impact of amygdalin on the proliferation, migration, and invasion of human endometrial stromal cells (HESCs) and the possible underlying mechanism. HESCs were incubated with 50, 100, and 200 µg/mL of amygdalin. The malignant activities of HESCs were analyzed by functional experiments. The activation of the Wnt/β-catenin signaling was tested using TOP/FOPFlash. The mRNA expressions of genes were validated by qRT-PCR. The endometriosis (EMS) mouse model was induced and the impact of amygdalin on the growth of ectopic endometrial lesions were assessed. It was observed that amygdalin markedly lessened the malignant activities of HESCs in a dose-dependent way (p < 0.05). Amygdalin dose-dependently declined the activation of TOPFlash and mRNA levels of β-catenin, cyclinD1 and c-Myc in HESCs (p < 0.05). Additionally, the increasing dose of amygdalin progressively inhibited the growth of ectopic endometrial lesions in EMS mouse model (p < 0.05). We reached a conclusion that amygdalin could inhibit the malignant activities of HESCs and alleviate EMS, which was related to Wnt/β-catenin signaling activation.

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杏仁苷通过抑制 Wnt/β-catenin 信号传导抑制子宫内膜异位症小鼠子宫内膜基质细胞的增殖、迁移和侵袭

探讨苦杏仁苷对人子宫内膜基质细胞（HESCs）增殖、迁移和侵袭的影响及其可能的内在机制。用 50、100 和 200 µg/mL 的苦杏仁苷培养 HESCs。通过功能实验分析了HESCs的恶性活性。使用TOP/FOPFlash测试了Wnt/β-catenin信号的激活情况。通过 qRT-PCR 验证了基因的 mRNA 表达。诱导子宫内膜异位症（EMS）小鼠模型，评估杏仁苷对异位子宫内膜病灶生长的影响。结果表明，苦杏仁苷以剂量依赖的方式显著降低了HESCs的恶性活性（p < 0.05）。杏仁苷剂量依赖性地降低了 TOPFlash 的活化以及 HESCs 中 β-catenin、cyclinD1 和 c-Myc 的 mRNA 水平（p < 0.05）。此外，增加杏仁苷的剂量可逐渐抑制 EMS 小鼠异位子宫内膜病变的生长（p < 0.05）。我们得出结论：苦杏仁苷能抑制HESCs的恶性活性，缓解EMS，这与Wnt/β-catenin信号激活有关。

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Triton X-100

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4% paraformaldehyde

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来源期刊

Journal of Molecular Histology 生物-细胞生物学

CiteScore

5.90

自引率

0.00%

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审稿时长

1 months

期刊介绍： The Journal of Molecular Histology publishes results of original research on the localization and expression of molecules in animal cells, tissues and organs. Coverage includes studies describing novel cellular or ultrastructural distributions of molecules which provide insight into biochemical or physiological function, development, histologic structure and disease processes. Major research themes of particular interest include: - Cell-Cell and Cell-Matrix Interactions; - Connective Tissues; - Development and Disease; - Neuroscience. Please note that the Journal of Molecular Histology does not consider manuscripts dealing with the application of immunological or other probes on non-standard laboratory animal models unless the results are clearly of significant and general biological importance. The Journal of Molecular Histology publishes full-length original research papers, review articles, short communications and letters to the editors. All manuscripts are typically reviewed by two independent referees. The Journal of Molecular Histology is a continuation of The Histochemical Journal.