Activity pattern of antioxidant enzymes in relation to the time of exposure of hexavalent chromium to Nile tilapia Oreochromis niloticus.

Abstract

Hexavalent chromium (Cr (VI)), a toxicant of environmental concern, frequently enters into water bodies and produces oxidative stress in fish. The antioxidant enzymes, Superoxide dismutase (SOD), Catalase (CAT), and Glutathion S-transferase (GST) are activated to counteract the oxidative stress in fish. This study explores the pattern of activation of these enzymes in gill, muscle, liver, and kidney tissues of Nile tilapia Oreochromis niloticus exposed to 9.35 mg/L and 18.70 mg/L of Cr (VI) for 96 h. The optimal hour of activity of these enzymes was revealed through extensive regression analysis. The results indicate a bell-shaped time response curve in the activity of the enzymes in both the treatments, except CAT in the gill of fish exposed to 18.70 mg/L Cr (VI) and GST in the gill, liver, and kidney of fish exposed to 18.70 mg/L Cr (VI). The results indicate that the optimal hour of activity of SOD changes in tandem with CAT, SOD responding first followed by CAT, both diminishing within 96 h. However, deviating from the bell-shaped pattern, the activity of CAT in gill and GST in gill, liver, and kidney in fish exposed to 18.70 mg/L Cr (VI) continued to rise even at 96 h, indicating that these antioxidant enzymes could not diminish the oxidative stress produced by the higher dose of Cr (VI). It was concluded that the activity of SOD, CAT, and GST between 30 and 70 h in the gill, liver, and kidney of Nile tilapia could serve as excellent biomarkers of oxidative stress under low doses of Cr (VI).