Pan-cancer analysis of IRF1 focusing on prognostic and immunological roles in non-small cell lung cancer.

IF 3.6 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Heliyon Pub Date : 2024-11-07 eCollection Date: 2024-11-30 DOI:10.1016/j.heliyon.2024.e39861

Weiling Sun, Kui Cao, Siran Wang, Mengdi Lu, Jianqun Ma, Chunlong Wu, Yanbin Zhao

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Abstract

Interferon regulatory factor 1 (IRF1) significantly affects tumour occurrence and development. This study aimed to analyse its function as a pan-cancer prognostic indicator. We compared IRF1 expression and prognostic significance in normal and tumour samples from different databases. Accordingly, we performed in vitro experiments and immunohistochemistry (IHC) to investigate the role of IRF1 in non-small cell lung cancer (NSCLC). Our findings indicate that IRF1 expression is significantly correlated with prognosis, the tumour microenvironment, and immune cell infiltration. Furthermore, receiver operating characteristic (ROC) analysis revealed that IRF1 had high accuracy in distinguishing cancerous tissues from normal ones. Notably, IRF1 expression was linked to immune-related and immune checkpoint genes. Cell proliferation, invasion, and migration were significantly related to IRF1 expression. IHC indicated that IRF1 was downregulated in NSCLC tissues. Our study provides comprehensive bioinformatic analysis and experimental verification of IRF1, suggesting its potential as a prognostic biomarker in cancer.

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对 IRF1 进行泛癌症分析，重点关注其在非小细胞肺癌中的预后和免疫学作用。

干扰素调节因子1（IRF1）对肿瘤的发生和发展有重大影响。本研究旨在分析其作为泛癌症预后指标的功能。我们比较了不同数据库中正常样本和肿瘤样本中 IRF1 的表达及其预后意义。因此，我们进行了体外实验和免疫组化（IHC），以研究 IRF1 在非小细胞肺癌（NSCLC）中的作用。我们的研究结果表明，IRF1的表达与预后、肿瘤微环境和免疫细胞浸润密切相关。此外，接受者操作特征（ROC）分析表明，IRF1 在区分癌组织和正常组织方面具有很高的准确性。值得注意的是，IRF1的表达与免疫相关基因和免疫检查点基因有关。细胞增殖、侵袭和迁移与 IRF1 的表达显著相关。IHC显示，IRF1在NSCLC组织中下调。我们的研究对IRF1进行了全面的生物信息学分析和实验验证，表明它有可能成为癌症预后的生物标志物。

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来源期刊

Heliyon MULTIDISCIPLINARY SCIENCES-

CiteScore

4.50

自引率

2.50%

发文量

2793

期刊介绍： Heliyon is an all-science, open access journal that is part of the Cell Press family. Any paper reporting scientifically accurate and valuable research, which adheres to accepted ethical and scientific publishing standards, will be considered for publication. Our growing team of dedicated section editors, along with our in-house team, handle your paper and manage the publication process end-to-end, giving your research the editorial support it deserves.