A novel process for transcellular hemoglobin transport from macrophages to cancer cells.

IF 8.2 2区生物学 Q1 CELL BIOLOGY Cell Communication and Signaling Pub Date : 2024-11-27 DOI:10.1186/s12964-024-01929-8

Agata Braniewska, Marcin Skorzynski, Zuzanna Sas, Magdalena Dlugolecka, Ilona Marszalek, Daria Kurpiel, Marcel Bühler, Damian Strzemecki, Aneta Magiera, Maciej Bialasek, Jaroslaw Walczak, Lukasz Cheda, Michal Komorowski, Tobias Weiss, Małgorzata Czystowska-Kuzmicz, Karina Kwapiszewska, Alberto Boffi, Magdalena Krol, Tomasz P Rygiel

{"title":"A novel process for transcellular hemoglobin transport from macrophages to cancer cells.","authors":"Agata Braniewska, Marcin Skorzynski, Zuzanna Sas, Magdalena Dlugolecka, Ilona Marszalek, Daria Kurpiel, Marcel Bühler, Damian Strzemecki, Aneta Magiera, Maciej Bialasek, Jaroslaw Walczak, Lukasz Cheda, Michal Komorowski, Tobias Weiss, Małgorzata Czystowska-Kuzmicz, Karina Kwapiszewska, Alberto Boffi, Magdalena Krol, Tomasz P Rygiel","doi":"10.1186/s12964-024-01929-8","DOIUrl":null,"url":null,"abstract":"<p><p>Hemoglobin (Hb) performs its physiological function within the erythrocyte. Extracellular Hb has prooxidative and proinflammatory properties and is therefore sequestered by haptoglobin and bound by the CD163 receptor on macrophages. In the present study, we demonstrate a novel process of Hb uptake by macrophages independent of haptoglobin and CD163. Unexpectedly, macrophages do not degrade the entire Hb, but instead transfer it to neighboring cells. We have shown that the phenomenon of Hb transfer from macrophages to other cells is mainly mediated by extracellular vesicles. In contrast to the canonical Hb degradation pathway by macrophages, Hb transfer has not been reported before. In addition, we have used the process of Hb transfer in anticancer therapy, where macrophages are loaded with a Hb-anticancer drug conjugate and act as cellular drug carriers. Both mouse and human macrophages loaded with Hb-monomethyl auristatin E (MMAE) effectively killed cancer cells when co-cultured in vitro.</p>","PeriodicalId":55268,"journal":{"name":"Cell Communication and Signaling","volume":"22 1","pages":"570"},"PeriodicalIF":8.2000,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Communication and Signaling","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s12964-024-01929-8","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Hemoglobin (Hb) performs its physiological function within the erythrocyte. Extracellular Hb has prooxidative and proinflammatory properties and is therefore sequestered by haptoglobin and bound by the CD163 receptor on macrophages. In the present study, we demonstrate a novel process of Hb uptake by macrophages independent of haptoglobin and CD163. Unexpectedly, macrophages do not degrade the entire Hb, but instead transfer it to neighboring cells. We have shown that the phenomenon of Hb transfer from macrophages to other cells is mainly mediated by extracellular vesicles. In contrast to the canonical Hb degradation pathway by macrophages, Hb transfer has not been reported before. In addition, we have used the process of Hb transfer in anticancer therapy, where macrophages are loaded with a Hb-anticancer drug conjugate and act as cellular drug carriers. Both mouse and human macrophages loaded with Hb-monomethyl auristatin E (MMAE) effectively killed cancer cells when co-cultured in vitro.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

从巨噬细胞到癌细胞的跨细胞血红蛋白运输新过程。

血红蛋白（Hb）在红细胞内发挥其生理功能。细胞外的 Hb 具有促氧化和促炎症的特性，因此会被血红蛋白封存，并与巨噬细胞上的 CD163 受体结合。在本研究中，我们展示了巨噬细胞摄取 Hb 的新过程，这一过程与血红蛋白和 CD163 无关。令人意想不到的是，巨噬细胞不会降解整个血红蛋白，而是将其转移到邻近的细胞中。我们已经证明，Hb 从巨噬细胞转移到其他细胞的现象主要是由细胞外囊泡介导的。与巨噬细胞典型的 Hb 降解途径不同，Hb 转移以前从未报道过。此外，我们还将 Hb 转移过程用于抗癌治疗，即巨噬细胞装载 Hb 抗癌药物共轭物并充当细胞药物载体。小鼠和人类巨噬细胞在体外共培养时，都负载了 Hb- 单甲基金丝桃素 E（MMAE），能有效杀死癌细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Cell Communication and Signaling CELL BIOLOGY-

CiteScore

11.00

自引率

0.00%

发文量

180

期刊介绍： Cell Communication and Signaling (CCS) is a peer-reviewed, open-access scientific journal that focuses on cellular signaling pathways in both normal and pathological conditions. It publishes original research, reviews, and commentaries, welcoming studies that utilize molecular, morphological, biochemical, structural, and cell biology approaches. CCS also encourages interdisciplinary work and innovative models, including in silico, in vitro, and in vivo approaches, to facilitate investigations of cell signaling pathways, networks, and behavior. Starting from January 2019, CCS is proud to announce its affiliation with the International Cell Death Society. The journal now encourages submissions covering all aspects of cell death, including apoptotic and non-apoptotic mechanisms, cell death in model systems, autophagy, clearance of dying cells, and the immunological and pathological consequences of dying cells in the tissue microenvironment.