Diosgenin Mediated JAK/STAT Signal Channel Improving SD Rat Renal Ischemia-Reperfusion.

Transplantation proceedings Pub Date : 2024-12-01 Epub Date: 2024-11-27 DOI:10.1016/j.transproceed.2024.11.007
Jinran Yang, Xinchang Li, Chengmei Long, Youfu Zhang, Hua Yang, Tongchang Chen, Xuyang Wang, Xiaomei Huang
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Abstract

Objective: To explore the mechanism of action of diosgenin and JAK/STAT in SD rat renal ischemia-reperfusion (I/R) injury.

Method: A renal I/R injury model was established, rat was divided into four groups: control group, sham surgery group, I/R model group, and diosgenin pretreatment group. 48 hours after the completion of modeling, blood was collected for testing of SCr, BUN, and Kim-1. After blood collection, bilateral kidneys were cut and stored in liquid nitrogen for HE staining. Western blot was used to detect the protein expression levels of JAK and STAT in each group. Compare and analyze the differences in JAK/STAT expression levels among different groups.

Result: Compared with the I/R model group, the Scr, Bun, and Kim-1 concentrations of the three treatment groups were significantly lower, the other three treatment groups showed significant improvement in symptoms of renal vacuolar degeneration and interstitial edema in rats, the expression levels of JAK and STAT in the three groups were significantly lower, while the difference between the three groups was not significant.

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薯蓣皂苷介导的 JAK/STAT 信号通道改善 SD 大鼠肾缺血再灌注。
目的探讨薯蓣皂苷与JAK/STAT在SD大鼠肾缺血再灌注损伤中的作用机制:方法:建立SD大鼠肾缺血再灌注损伤模型,将大鼠分为四组:对照组、假手术组、I/R模型组和地奥司宁预处理组。模型制作完成 48 小时后,采血检测 SCr、BUN 和 Kim-1。采血后,切取双侧肾脏并保存在液氮中进行 HE 染色。用 Western blot 检测各组 JAK 和 STAT 的蛋白表达水平。比较并分析不同组间 JAK/STAT 表达水平的差异:与I/R模型组相比,三个治疗组的Scr、Bun和Kim-1浓度明显降低,其他三个治疗组大鼠肾空泡变性和间质水肿症状明显改善,三个治疗组的JAK和STAT表达水平明显降低,三组间差异无显著性。
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