David Eduardo Prado-Tarango , Ricardo Mata-González , Dana Sanchez , Lisa Ellsworth
{"title":"Diversity and Abundance of Arbuscular Mycorrhizal Fungi and Soil Bacteria in Purshia tridentata Stands in the Sagebrush Steppe","authors":"David Eduardo Prado-Tarango , Ricardo Mata-González , Dana Sanchez , Lisa Ellsworth","doi":"10.1016/j.rama.2024.06.013","DOIUrl":null,"url":null,"abstract":"<div><div>Soil microorganisms, including arbuscular mycorrhizal fungi (AMF) and nitrogen fixing bacteria, require better integration into ecological restoration programs as they might facilitate plant establishment. Both are believed to be critical for the establishment of native rangeland plant species such as <em>Purshia tridentata</em>. We evaluated the AMF and bacterial diversity and abundance from soils in 1) areas with undisturbed P. tridentata stands in rangelands of north-central Oregon and 2) areas where <em>P. tridentata</em> was eliminated because of wildfires. We collected soil from three locations 1) areas with undisturbed <em>P. tridentata</em> stands in rangelands of North-central Oregon and 2) areas where <em>P. tridentata</em> was eliminated because of wildfires. We also collected roots from live <em>P tridentata</em> plants<em>.</em> We used 16s rRNA gene and ITS amplicon sequencing to determine and compare bacterial and AMF microbiomes. We wanted to evaluate the soil bacteria to the family level to determine the presence of the Frankiaceae family, and the AMF to the species level to evaluate which AMF species colonized this plant. Bacterial and AMF diversity did not vary between locations, but diversity and abundance of both parameters differed in roots (<em>P</em> < 0.05) from those of soils. AMF abundance in roots was dominated by <em>Claroideoglomus</em> and <em>Glomus</em>. The major bacterial familiy detected in soil samples was Chthoniobacteraceae, while Frankiaceae was present to a very low extent. Our results suggest that current microbial communities in the study area are homogeneous instead of being affected by the elimination of <em>P. tridentata</em>. However, our data reveals the current mycorrhizal communities associated with <em>P. tridentata</em> which can be used for restoration purposes.</div></div>","PeriodicalId":49634,"journal":{"name":"Rangeland Ecology & Management","volume":"98 ","pages":"Pages 18-27"},"PeriodicalIF":2.4000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rangeland Ecology & Management","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1550742424001027","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Soil microorganisms, including arbuscular mycorrhizal fungi (AMF) and nitrogen fixing bacteria, require better integration into ecological restoration programs as they might facilitate plant establishment. Both are believed to be critical for the establishment of native rangeland plant species such as Purshia tridentata. We evaluated the AMF and bacterial diversity and abundance from soils in 1) areas with undisturbed P. tridentata stands in rangelands of north-central Oregon and 2) areas where P. tridentata was eliminated because of wildfires. We collected soil from three locations 1) areas with undisturbed P. tridentata stands in rangelands of North-central Oregon and 2) areas where P. tridentata was eliminated because of wildfires. We also collected roots from live P tridentata plants. We used 16s rRNA gene and ITS amplicon sequencing to determine and compare bacterial and AMF microbiomes. We wanted to evaluate the soil bacteria to the family level to determine the presence of the Frankiaceae family, and the AMF to the species level to evaluate which AMF species colonized this plant. Bacterial and AMF diversity did not vary between locations, but diversity and abundance of both parameters differed in roots (P < 0.05) from those of soils. AMF abundance in roots was dominated by Claroideoglomus and Glomus. The major bacterial familiy detected in soil samples was Chthoniobacteraceae, while Frankiaceae was present to a very low extent. Our results suggest that current microbial communities in the study area are homogeneous instead of being affected by the elimination of P. tridentata. However, our data reveals the current mycorrhizal communities associated with P. tridentata which can be used for restoration purposes.
期刊介绍:
Rangeland Ecology & Management publishes all topics-including ecology, management, socioeconomic and policy-pertaining to global rangelands. The journal''s mission is to inform academics, ecosystem managers and policy makers of science-based information to promote sound rangeland stewardship. Author submissions are published in five manuscript categories: original research papers, high-profile forum topics, concept syntheses, as well as research and technical notes.
Rangelands represent approximately 50% of the Earth''s land area and provision multiple ecosystem services for large human populations. This expansive and diverse land area functions as coupled human-ecological systems. Knowledge of both social and biophysical system components and their interactions represent the foundation for informed rangeland stewardship. Rangeland Ecology & Management uniquely integrates information from multiple system components to address current and pending challenges confronting global rangelands.