Attenuating Cartilage Degeneration in a Low Mechanical Compression Rat Model Through Intra-Articular Injections of Allogeneic Bone Marrow-Derived Mesenchymal Stem Cells.

IF 2.7 4区医学 Q1 ORTHOPEDICS CARTILAGE Pub Date : 2024-11-29 DOI:10.1177/19476035241301291

Chia Tai, Akira Ito, Zixi Zhao, Hiroshi Kuroki, Tomoki Aoyama

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Abstract

Objective: Mechanical stimulation significantly contributes to posttraumatic osteoarthritis (PTOA), a condition that impedes patient recovery following intra-articular injury. Effective treatment options for compression-induced injuries are limited. Bone marrow-derived mesenchymal stem cell (BMSC) implantation has emerged as a potential therapeutic breakthrough for joint diseases. The aim of this study was to attenuate the progression of PTOA induced by cyclic loading and demonstrate the potential effectiveness of BMSCs in a rat model of low mechanical compression.

Design: Using a rat model of compression-induced articular cartilage injury, assessments were conducted 2, 4, and 8 weeks after cyclic compressive loading. The expression of matrix metallopeptidase 13, transforming growth factor-beta 3 (TGF-β3), insulin-like growth factor 1 (IGF-1), and cleaved caspase-3 was evaluated through immunohistochemistry to investigate the mechanistic aspects underlying the prevention of compression-induced injury following BMSCs treatment.

Results: Intra-articular injections of BMSCs significantly improved scores in the OARSI (Osteoarthritis Research Society International) Osteoarthritis Cartilage Histopathology Assessment System and Histological-Histochemical Grading System. This treatment showed positive outcomes in maintaining high relative cell density and reducing proteoglycan loss after cyclic compression-induced injury. The expression patterns of IGF-1 and TGF-β3 provide valuable insights into the presence and distribution of these growth factors in healthy and injured cartilage.

Conclusions: These findings highlight the efficacy of BMSCs treatment in attenuating the advancement of compression-induced injuries, albeit within a limited timeframe.

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通过关节内注射同种异体骨髓间充质干细胞减轻低机械压迫大鼠软骨退变模型。

目的：机械刺激对创伤后骨关节炎（PTOA）有显著的促进作用，这是一种阻碍患者在关节内损伤后恢复的疾病。压迫性损伤的有效治疗选择是有限的。骨髓间充质干细胞（BMSC）移植已成为关节疾病治疗的潜在突破。本研究的目的是减弱由循环载荷引起的PTOA的进展，并在低机械压缩大鼠模型中证明骨髓间充质干细胞的潜在有效性。设计：采用挤压性关节软骨损伤大鼠模型，在循环压缩加载2、4和8周后进行评估。通过免疫组织化学检测基质金属肽酶13、转化生长因子-β3 （TGF-β3）、胰岛素样生长因子1 （IGF-1）和cleaved caspase-3的表达，探讨骨髓间充质干细胞治疗后预防压迫性损伤的机制。结果：关节内注射骨髓间充质干细胞可显著提高OARSI （Osteoarthritis Research Society International）骨关节炎软骨组织病理学评估系统和组织组织化学分级系统的评分。这种治疗在维持较高的相对细胞密度和减少循环压缩损伤后的蛋白多糖损失方面显示出积极的结果。IGF-1和TGF-β3的表达模式为了解这些生长因子在健康和损伤软骨中的存在和分布提供了有价值的见解。结论：这些发现强调了骨髓间充质干细胞治疗在减轻压迫性损伤进展方面的有效性，尽管在有限的时间内。

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来源期刊

CARTILAGE ORTHOPEDICS-

CiteScore

6.90

自引率

7.10%

发文量

期刊介绍： CARTILAGE publishes articles related to the musculoskeletal system with particular attention to cartilage repair, development, function, degeneration, transplantation, and rehabilitation. The journal is a forum for the exchange of ideas for the many types of researchers and clinicians involved in cartilage biology and repair. A primary objective of CARTILAGE is to foster the cross-fertilization of the findings between clinical and basic sciences throughout the various disciplines involved in cartilage repair. The journal publishes full length original manuscripts on all types of cartilage including articular, nasal, auricular, tracheal/bronchial, and intervertebral disc fibrocartilage. Manuscripts on clinical and laboratory research are welcome. Review articles, editorials, and letters are also encouraged. The ICRS envisages CARTILAGE as a forum for the exchange of knowledge among clinicians, scientists, patients, and researchers. The International Cartilage Repair Society (ICRS) is dedicated to promotion, encouragement, and distribution of fundamental and applied research of cartilage in order to permit a better knowledge of function and dysfunction of articular cartilage and its repair.