A novel BODIPY-derived colorimetric and ratiometric dual-mode fluorescent probe for highly sensitive and visual detection of sulfite in food and living organisms

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS Analytical and Bioanalytical Chemistry Pub Date : 2024-11-29 DOI:10.1007/s00216-024-05658-2

Chunjie Zhang, Yueyin Liang, Shuai Gong, Zhiyuan Meng, Zhonglong Wang, Shifa Wang

{"title":"A novel BODIPY-derived colorimetric and ratiometric dual-mode fluorescent probe for highly sensitive and visual detection of sulfite in food and living organisms","authors":"Chunjie Zhang, Yueyin Liang, Shuai Gong, Zhiyuan Meng, Zhonglong Wang, Shifa Wang","doi":"10.1007/s00216-024-05658-2","DOIUrl":null,"url":null,"abstract":"<div><p>Sulfite, widely used as a food additive, performs indispensable functions in the field of food sterilization, bleaching, and antisepsis. However, the overuse of sulfite may destroy food nutrition and pose health risks to people. In this work, an innovative BODIPY-based fluorescent probe (<b>BODIPY-DBC</b>) was constructed for highly sensitive recognition of sulfite. The <b>BODIPY-DBC</b> probe possessed both colorimetric and ratiometric dual mode, a low detection limit (33.12 nM), high sensitivity, a wide pH usage range (5–12), a fast response time (2 min), and superior fluorescence imaging capability for detecting sulfite. The recognition mechanism was certificated by <sup>1</sup>H NMR titration, HRMS analysis, and DFT calculation. The <b>BODIPY-DBC</b> probe was not only loaded on test strips for detecting sulfite conveniently with the naked eye, but also employed to detect sulfite content in real food samples to ensure food safety. Furthermore, it also achieved excellent performances for monitoring sulfite in dual-channel fluorescence imaging (HeLa cell and zebrafish).</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":462,"journal":{"name":"Analytical and Bioanalytical Chemistry","volume":"417 2","pages":"405 - 415"},"PeriodicalIF":3.8000,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical and Bioanalytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00216-024-05658-2","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

Abstract

Sulfite, widely used as a food additive, performs indispensable functions in the field of food sterilization, bleaching, and antisepsis. However, the overuse of sulfite may destroy food nutrition and pose health risks to people. In this work, an innovative BODIPY-based fluorescent probe (BODIPY-DBC) was constructed for highly sensitive recognition of sulfite. The BODIPY-DBC probe possessed both colorimetric and ratiometric dual mode, a low detection limit (33.12 nM), high sensitivity, a wide pH usage range (5–12), a fast response time (2 min), and superior fluorescence imaging capability for detecting sulfite. The recognition mechanism was certificated by ¹H NMR titration, HRMS analysis, and DFT calculation. The BODIPY-DBC probe was not only loaded on test strips for detecting sulfite conveniently with the naked eye, but also employed to detect sulfite content in real food samples to ensure food safety. Furthermore, it also achieved excellent performances for monitoring sulfite in dual-channel fluorescence imaging (HeLa cell and zebrafish).

Graphical Abstract

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

一种新型的bodipy衍生的比色和比例双模荧光探针，用于高灵敏度和视觉检测食品和生物中的亚硫酸盐。

亚硫酸盐作为一种广泛使用的食品添加剂，在食品杀菌、漂白、防腐等方面发挥着不可缺少的作用。然而，亚硫酸盐的过度使用可能会破坏食物营养，并对人们的健康构成威胁。在这项工作中，一个创新的基于bodipy的荧光探针（BODIPY-DBC）被构建为亚硫酸盐的高灵敏度识别。BODIPY-DBC探针具有比色和比例双模式，检测限低（33.12 nM），灵敏度高，pH使用范围宽（5-12），响应时间快（2 min），并且具有检测亚硫酸盐的优异荧光成像能力。通过1H NMR滴定、HRMS分析和DFT计算验证了其识别机理。BODIPY-DBC探针不仅可以装在试纸上，方便肉眼检测亚硫酸盐，还可以用于检测真实食品样品中的亚硫酸盐含量，确保食品安全。此外，在双通道荧光成像（HeLa细胞和斑马鱼）中监测亚硫酸盐也取得了优异的性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Analytical and Bioanalytical Chemistry 化学-分析化学

CiteScore

8.00

自引率

4.70%

发文量

638

审稿时长

2.1 months

期刊介绍： Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.