Isolation and identification of aerobic and anaerobic bacteria from the feces of wild Korean water deer (Hydropotes inermis argyropus).

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES Journal of Veterinary Science Pub Date : 2024-11-01 DOI:10.4142/jvs.24236
Md Ashraful Islam, Sungryong Kim, Md Sodrul Islam, Obaidul Islam, Seunghyeon Park, Itainara Taili, Dong-Hyuk Jeong, Ki-Jeong Na
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引用次数: 0

Abstract

Importance: Understanding the microbial diversity within the gastrointestinal tract of wild Korean water deer (KWD; Hydropotes inermis argyropus) is essential for gaining insights into their health and ecological interactions.

Objective: This study aims to isolate and identify aerobic and anaerobic bacterial species in the feces of wild KWD.

Methods: Fecal samples were collected from 55 wild KWD of varying age and sex. Aerobic bacteria were cultured at 37°C for 24-48 h under standard conditions, whereas anaerobic bacteria were cultured at 37°C for 48-72 h in an anaerobic environment. Bacterial identification was conducted using DNA extraction and polymerase chain reaction amplification targeting the 16S rRNA gene.

Results: The predominant aerobic bacteria identified belonged to the Firmicutes (58.18%) and Proteobacteria (41.82%) phyla, with Escherichia coli (31.82%) and Bacillus cereus (31.82%) being the most common species. Among anaerobic bacteria, most belonged to the Firmicutes (71.03%), Proteobacteria (27.10%), and Fusobacteriota (1.87%) phyla, with Paraclostridium bifermentans (28.97%) and E. coli (22.43%) being the most prevalent species. Other frequently identified anaerobic species were Fusobacterium varium, Lactococcus garvieae, Terrisporobacter glycolicus, Enterococcus faecalis, and Clostridium sporogenes.

Conclusions and relevance: Our findings indicate a diverse microbial community in the feces of water deer, offering valuable insights into their gut microbiota and its potential implications for health and ecology.

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来源期刊
Journal of Veterinary Science
Journal of Veterinary Science 农林科学-兽医学
CiteScore
3.10
自引率
5.60%
发文量
86
审稿时长
1.3 months
期刊介绍: The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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