Cytoprotective Properties of Turmeric Oil (Curcuma longa L.) on Fibroblast Cells.

Q2 Medicine Asian Pacific Journal of Cancer Prevention Pub Date : 2024-11-01 DOI:10.31557/APJCP.2024.25.11.4005

Riris Istighfari Jenie, Anif Nur Artanti, Rumiyati Rumiyati, Dhania Novitasari, Yuni Kusumastuti, Manami Toriyama

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Abstract

Objective: Senescence is a cellular physiological process involved in cell aging. One factor that increases senescence is oxidative stress, which can be induced by hydrogen peroxide. Active compounds in turmeric (Curcuma longa) are classified as volatile and non-volatile. Major non-volatile compounds in turmeric are curcumin, dimethoxy curcumin, and bisdemethoxycurcumin bioactivities that have been widely explored. However, turmeric rhizome oil (TO) has limited reports on its bioactivity and constituents. This study aims to determine the potency of TO as cytoprotective against oxidative stress induced by hydrogen peroxide using the fibroblast cell lines (NIH-3T3 and HDF).

Methods: We evaluated the cytotoxicity of TO using MTT assay, then evaluated its effect on cell senescence using SA-β-gal assay. The cellular reactive oxygen species (ROS) level was observed using DCFDA staining through flow cytometry. The turmeric volatile oil which was obtained by steam-water distillation was analyzed with a gas chromatography-mass spectrophotometry (GC-MS) to determine the chemical profile.

Results: TO showed low cytotoxicity against HDF and NIH-3T3 cells, with IC50 values of over 100 µM. TO rescued cells from undergoing senescence and reduced ROS levels which were induced by hydrogen peroxide. The GC-MS spectra of the TO compound in positive ionization mode showed retention times of 23.56 and 26.20 minutes, corresponding to the ar-turmerone and turmerone compounds.

Conclusion: These results indicated that TO has the potency as a cytoprotective agent in stress oxidative conditions.

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姜黄油对成纤维细胞的保护作用。

目的：衰老是一种涉及细胞衰老的细胞生理过程。导致衰老的一个因素是氧化应激，它可以由过氧化氢引起。姜黄中的活性化合物分为挥发性和非挥发性。姜黄中主要的非挥发性化合物是姜黄素、二甲氧基姜黄素和双去甲氧基姜黄素，其生物活性已被广泛研究。然而，姜黄根茎油（TO）的生物活性和成分的报道有限。本研究旨在利用成纤维细胞系（NIH-3T3和HDF）确定to对过氧化氢诱导的氧化应激的细胞保护作用。方法：采用MTT法评价TO的细胞毒性，采用SA-β-gal法评价TO对细胞衰老的影响。流式细胞术DCFDA染色观察细胞活性氧（ROS）水平。采用气相色谱-质谱联用技术对蒸汽-水蒸馏法提取的姜黄挥发油进行化学分析。结果：TO对HDF和NIH-3T3细胞具有较低的细胞毒性，IC50值均在100µM以上。TO可使细胞免于衰老，降低过氧化氢诱导的ROS水平。在正电离模式下，TO化合物的GC-MS谱显示其保留时间分别为23.56和26.20分钟，对应于ar-turmerone和turmerone化合物。结论：TO在应激氧化条件下具有一定的细胞保护剂作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Asian Pacific Journal of Cancer Prevention 医学-肿瘤学

CiteScore

2.80

自引率

0.00%

发文量

779

审稿时长

3 months

期刊介绍： Cancer is a very complex disease. While many aspects of carcinoge-nesis and oncogenesis are known, cancer control and prevention at the community level is however still in its infancy. Much more work needs to be done and many more steps need to be taken before effective strategies are developed. The multidisciplinary approaches and efforts to understand and control cancer in an effective and efficient manner, require highly trained scientists in all branches of the cancer sciences, from cellular and molecular aspects to patient care and palliation. The Asia Pacific Organization for Cancer Prevention (APOCP) and its official publication, the Asia Pacific Journal of Cancer Prevention (APJCP), have served the community of cancer scientists very well and intends to continue to serve in this capacity to the best of its abilities. One of the objectives of the APOCP is to provide all relevant and current scientific information on the whole spectrum of cancer sciences. They aim to do this by providing a forum for communication and propagation of original and innovative research findings that have relevance to understanding the etiology, progression, treatment, and survival of patients, through their journal. The APJCP with its distinguished, diverse, and Asia-wide team of editors, reviewers, and readers, ensure the highest standards of research communication within the cancer sciences community across Asia as well as globally. The APJCP publishes original research results under the following categories: -Epidemiology, detection and screening. -Cellular research and bio-markers. -Identification of bio-targets and agents with novel mechanisms of action. -Optimal clinical use of existing anti-cancer agents, including combination therapies. -Radiation and surgery. -Palliative care. -Patient adherence, quality of life, satisfaction. -Health economic evaluations.